Surinkaew Sirirat, Mongkolpathumrat Podsawee, Nissapatorn Veeranoot, Kumphune Sarawut
Research Excellence Center for Innovation and Health Products (RECIHP), Walailak University, Nakhon Si Thammarat, 80160, Thailand.
School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat, 80160, Thailand.
F1000Res. 2022 Oct 31;11:1235. doi: 10.12688/f1000research.126051.1. eCollection 2022.
Ischemic heart disease is a leading cause of death in patients with cardiovascular disease. Natural products containing high antioxidant activity have been used as an alternative therapy to improve the living conditions of patients. In this study, we examine the protective effect of tamarind seed (TS) on myocardial hypoxic injury. The hypoxia model was mimicked by mineral oil overlayed on H9c2 cardiomyoblasts for 4 h. TS extract was pretreated and administered during the hypoxic condition. Radical scavenging activity of TS extract was measured and exhibited very potent antioxidant activities on 2,2-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assays. TS extract at a concentration of 10 µg/ml significantly reversed the effect of hypoxia-induced cell death and intracellular reactive oxygen species (ROS) production. We also observed hypoxia-induced over-expression of both inflammatory cytokine mRNA and activation of cellular apoptosis. Pretreatment of TS extract significantly reduced hypoxia-induced HIF-1a and pro-inflammatory cytokine production, IL-1b and IL-6. The Western blot analysis for apoptotic regulatory molecules, caspase 3, caspase 8 and Bax proteins, also showed hypoxia injury reversal by TS extract treatment. The results suggest that the anti-ischemic effect of TS extract protects against hypoxia-induced injury and has potential to be an effective alternative therapy for ischemic heart disease and oxidative-damage related disease.
缺血性心脏病是心血管疾病患者死亡的主要原因。含有高抗氧化活性的天然产物已被用作改善患者生活条件的替代疗法。在本研究中，我们研究了罗望子种子（TS）对心肌缺氧损伤的保护作用。通过在H9c2心肌成纤维细胞上覆盖矿物油4小时来模拟缺氧模型。在缺氧条件下对TS提取物进行预处理并给药。测定了TS提取物的自由基清除活性，其在2,2-二苯基-2-苦基肼（DPPH）和2,2'-偶氮双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）测定中表现出非常强的抗氧化活性。浓度为10μg/ml的TS提取物显著逆转了缺氧诱导的细胞死亡和细胞内活性氧（ROS）产生的影响。我们还观察到缺氧诱导的炎性细胞因子mRNA的过度表达和细胞凋亡的激活。TS提取物的预处理显著降低了缺氧诱导的HIF-1α和促炎细胞因子的产生，即IL-1β和IL-6。对凋亡调节分子caspase 3、caspase 8和Bax蛋白的蛋白质印迹分析也表明，TS提取物处理可逆转缺氧损伤。结果表明，TS提取物的抗缺血作用可保护免受缺氧诱导的损伤，并且有可能成为缺血性心脏病和氧化损伤相关疾病的有效替代疗法。
