Guzmán María Camila, de Vivero María, Gil Isabel, Marrugo Victoria, Mercado Dilia, Zakzuk Josefina, Puerta Leonardo, Acevedo Nathalie, Caraballo Luis
Instituto de Investigaciones Inmunológicas, Universidad de Cartagena, Cartagena, Colombia.
Clínica Respiratoria y de Alergias, Cartagena, Colombia.
Rev Alerg Mex. 2024 Feb 1;71(1):74. doi: 10.29262/ram.v71i1.1362.
Determine the main asthma phenotypes in a population of asthmatic children in Cartagena, Colombia.
107 children (7 to 17 years old) with a previous diagnosis of asthma were recruited. Biomarkers of T2 inflammation were evaluated by measuring FeNO, eosinophil count in peripheral blood by hemocytometry, and determination of specific IgE to mite allergens by ELISA. The study was approved by the ethics committee of the University of Cartagena (SGR, Grant BPIN2020000100405).
The average age of patients was 10,9 years. 19,6% of the children did not show elevation of any of the T2 inflammation biomarkers evaluated (FeNO<20ppb, eos<300/ul, negative specific IgE), so they were considered patients with non-allergic asthma (non-T2). 71,9% of all patients were sensitized to at least one allergen, this phenotype was considered allergic asthma. 30,8% of the patients presented the three elevated biomarkers (FeNO>20ppb + eos >300/ul + positive specific IgE), this phenotype was classified as high T2 allergic asthma. A moderate correlation (Spearman rho=0,44, p<0,0001) was found between FeNO values and eosinophil counts.
In this study, the following phenotypes were found: allergic asthma, high T2 asthma, and non-allergic asthma. Most patients presented a type 2 inflammatory phenotype with allergic sensitization. In addition to the measurement of specific IgE, the use of FeNO and eosinophil count in peripheral blood help to accurately determine those patients with high T2 asthma phenotypes.
确定哥伦比亚卡塔赫纳哮喘儿童群体中的主要哮喘表型。
招募了107名先前诊断为哮喘的儿童（7至17岁）。通过测量呼出一氧化氮（FeNO）、采用血细胞计数法检测外周血嗜酸性粒细胞计数以及用酶联免疫吸附测定法（ELISA）测定对螨过敏原的特异性IgE来评估2型炎症生物标志物。该研究获得了卡塔赫纳大学伦理委员会的批准（SGR，资助编号BPIN2020000100405）。
患者的平均年龄为10.9岁。19.6%的儿童未表现出所评估的任何2型炎症生物标志物升高（FeNO<20ppb，嗜酸性粒细胞<300/μl，特异性IgE阴性），因此他们被视为非过敏性哮喘（非2型）患者。所有患者中有71.9%对至少一种过敏原致敏，该表型被视为过敏性哮喘。30.8%的患者呈现出三种生物标志物升高（FeNO>20ppb + 嗜酸性粒细胞>300/μl + 特异性IgE阳性），该表型被归类为高2型过敏性哮喘。发现FeNO值与嗜酸性粒细胞计数之间存在中度相关性（Spearman秩相关系数=0.44，p<0.0001）。
在本研究中，发现了以下表型：过敏性哮喘、高2型哮喘和非过敏性哮喘。大多数患者呈现出伴有过敏致敏的2型炎症表型。除了测量特异性IgE外，使用FeNO和外周血嗜酸性粒细胞计数有助于准确确定那些具有高2型哮喘表型的患者。
