Department of Clinical Oncology, Harbin Medical University Cancer Hospital, 150 Haping Road, Harbin, 150040, China.
Clin Exp Med. 2024 Apr 29;24(1):89. doi: 10.1007/s10238-024-01356-6.
The significance of Protein phosphatase 4 catalytic subunit (PPP4C) in diffuse large B-cell lymphoma (DLBCL) prognosis is not well understood. This work aimed to investigate the expression of PPP4C in DLBCL, investigate the correlation between PPP4C expression and clinicopathological parameters, and assess the prognostic significance of PPP4C. The mRNA expression of PPP4C was investigated using data from TCGA and GEO. To further analyze PPP4C expression, immunohistochemistry was performed on tissue microarray samples. Correlation analysis between clinicopathological parameters and PPP4C expression was conducted using Pearson's chi-square test or Fisher's exact test. Univariate and multivariate Cox hazard models were utilized to determine the prognostic significance of clinicopathological features and PPP4C expression. Additionally, survival analysis was performed using Kaplan-Meier survival curves. In both TCGA and GEO datasets, we identified higher mRNA levels of PPP4C in tumor tissues compared to normal tissues. Upon analysis of various clinicopathological features of DLBCL, we observed a correlation between high PPP4C expression and ECOG score (P = 0.003). Furthermore, according to a Kaplan-Meier survival analysis, patients with DLBCL who exhibit high levels of PPP4C had worse overall survival (P = 0.001) and progression-free survival (P = 0.002). PPP4C was shown to be an independent predictive factor for OS and PFS in DLBCL by univariate and multivariate analysis (P = 0.011 and P = 0.040). This study's findings indicate that high expression of PPP4C is linked to a poor prognosis for DLBCL and may function as an independent prognostic factors.
蛋白磷酸酶 4 催化亚基（PPP4C）在弥漫性大 B 细胞淋巴瘤（DLBCL）预后中的意义尚不清楚。本研究旨在探讨 PPP4C 在 DLBCL 中的表达，分析 PPP4C 表达与临床病理参数的相关性，并评估 PPP4C 的预后意义。通过 TCGA 和 GEO 数据库分析 PPP4C 的 mRNA 表达。为了进一步分析 PPP4C 的表达，对组织微阵列样本进行免疫组织化学染色。采用 Pearson 卡方检验或 Fisher 确切概率法分析 PPP4C 表达与临床病理参数的相关性。采用单因素和多因素 Cox 风险模型分析临床病理特征和 PPP4C 表达的预后意义。此外，还采用 Kaplan-Meier 生存曲线进行生存分析。在 TCGA 和 GEO 数据集均发现肿瘤组织中 PPP4C 的 mRNA 水平高于正常组织。对 DLBCL 的各种临床病理特征进行分析，发现 PPP4C 高表达与 ECOG 评分相关（P=0.003）。Kaplan-Meier 生存分析显示，PPP4C 高表达的 DLBCL 患者总生存（P=0.001）和无进展生存（P=0.002）更差。单因素和多因素分析显示，PPP4C 是 DLBCL 患者 OS 和 PFS 的独立预测因素（P=0.011 和 P=0.040）。本研究结果表明，PPP4C 高表达与 DLBCL 预后不良相关，可能是独立的预后因素。
