Counter WMD Systems, Massachusetts Institute of Technology, Lincoln Laboratory, Lexington, MA, USA.
Research & Support Unit, Laboratory Division, Federal Bureau of Investigation, Quantico, VA, USA.
Methods Mol Biol. 2024;2744:391-402. doi: 10.1007/978-1-0716-3581-0_25.
This chapter describes procedures for the use of DNA sequence data to obtain and compare taxonomic identification using the public databases GenBank and Barcode of Life Data System (BOLD). The chapter begins by describing procedures used to prepare quality sequences for uploading into GenBank and BOLD. Next, steps used to query the DNA sequences against the public databases are described using GenBank BLAST and BOLD identification engines. Interpretation guidelines for the taxonomic identification assignments are presented. Finally, a procedure for evaluating the accuracy and reliability of sequences from GenBank and BOLD is provided.
本章描述了使用 DNA 序列数据通过公共数据库 GenBank 和生命条形码数据系统（BOLD）获取和比较分类鉴定的过程。本章首先描述了为将高质量序列上传到 GenBank 和 BOLD 而进行的准备过程。接下来，描述了使用 GenBank BLAST 和 BOLD 鉴定引擎查询公共数据库中的 DNA 序列的步骤。还介绍了分类鉴定结果的解释指南。最后，提供了一种评估 GenBank 和 BOLD 序列准确性和可靠性的方法。
