Drexler H G, Klein M, Bhoopalam N, Menon M, Messmore H L, Gaedicke G, Minowada J
Eur J Cancer Clin Oncol. 1985 Dec;21(12):1485-91. doi: 10.1016/0277-5379(85)90243-3.
The carboxylic esterase (E.C. 3.1.1.1) isoenzymes from cases of acute myeloid leukemias were separated by analytical isoelectric focusing on horizontal thin-layer gels. One isoenzyme consisting of one or two components (bands) could be completely and selectively inhibited by addition of 40 mM sodium fluoride (NaF) to the staining bath. The 105 cases were classified into the groups M1-M6 according to the FAB proposals. The NaF-sensitive isoenzyme was not detected in cases of FAB groups M1/2 (acute myeloblastic leukemia without or with maturation), group M3 (acute promyelocytic leukemia) or group M6 (erythroleukemia). Thirty-one out of 33 cases in the FAB group M4 (acute myelomonocytic leukemia) and 9/9 cases in FAB group M5 (acute monocytic leukemia) expressed the NaF-sensitive isoenzyme. The NaF-sensitive isoenzyme was found at different staining intensities; all M5 cases showed the isoenzyme at strong or very strong intensity, whereas most of the M4 cases displayed the isoenzyme at weak, medium or strong staining intensity. The data presented are further evidence that the presence of the NaF-sensitive esterase isoenzyme indicates monocytic involvement or differentiation in cases of myeloid leukemias. The easy and fast to perform method of isoelectric focusing can be used to distinguish the monocytic variants among the acute myeloid leukemias and can supplement the morphological analysis of these cases.
通过在水平薄层凝胶上进行分析性等电聚焦，分离了急性髓系白血病患者的羧酸酯酶（E.C. 3.1.1.1）同工酶。向染色浴中加入40 mM氟化钠（NaF）可完全且选择性地抑制一种由一个或两个组分（条带）组成的同工酶。根据FAB建议，将105例患者分为M1 - M6组。在FAB组M1/2（无成熟或有成熟的急性髓性白血病）、M3组（急性早幼粒细胞白血病）或M6组（红白血病）患者中未检测到NaF敏感同工酶。FAB组M4（急性粒单核细胞白血病）的33例患者中有31例以及FAB组M5（急性单核细胞白血病）的9/9例患者表达了NaF敏感同工酶。发现NaF敏感同工酶的染色强度不同；所有M5病例的同工酶染色强度为强或非常强，而大多数M4病例的同工酶染色强度为弱、中等或强。所呈现的数据进一步证明，在髓系白血病病例中，NaF敏感酯酶同工酶的存在表明有单核细胞参与或分化。等电聚焦这种简便快速的方法可用于区分急性髓系白血病中的单核细胞变异型，并可补充这些病例的形态学分析。
