State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China.
School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, 230026, P. R. China.
Anal Chem. 2024 May 14;96(19):7353-7359. doi: 10.1021/acs.analchem.4c00266. Epub 2024 May 1.
Accurate detection of multiple cardiovascular biomarkers is crucial for the timely screening of acute coronary syndrome (ACS) and differential diagnosis from acute aortic syndrome (AAS). Herein, an antibody microarray-based metal-enhanced fluorescence assay (AMMEFA) has been developed to quantitatively detect 7 cardiovascular biomarkers through the formation of a sandwich immunoassay on the poly(glycidyl methacrylate--2-hydroxyethyl methacrylate)-decorated GNR-modified slide (GNR@P(GMA-HEMA) slide). The AMMEFA exhibits high specificity and sensitivity, the linear ranges span 5 orders of magnitude, and the limits of detection (LODs) of cardiac troponin I (cTnI), heart-type fatty acid binding protein (H-FABP), C-reactive protein (CRP), copeptin, myoglobin, D-Dimer, and N-terminal pro-brain natriuretic peptide (NT-proBNP) reach 0.07, 0.2, 65.7, 0.6, 0.2, 8.3, and 0.3 pg mL, respectively. To demonstrate its practicability, the AMMEFA has been applied to quantitatively analyze 7 cardiovascular biomarkers in 140 clinical plasma samples. In addition, the expression levels of cardiovascular biomarkers were analyzed by the least absolute shrinkage and selector operator (LASSO) regression, and the area under receiver operator characteristic curves (AUCs) of healthy donors (HDs), ACS patients, and AAS patients are 0.99, 0.98, and 0.97, respectively.
准确检测多种心血管生物标志物对于急性冠状动脉综合征（ACS）的及时筛查和与急性主动脉综合征（AAS）的鉴别诊断至关重要。在此，开发了一种基于抗体微阵列的金属增强荧光分析（AMMEFA），通过在聚（甲基丙烯酸缩水甘油酯-2-羟乙基甲基丙烯酸酯）修饰的 GNR 修饰载玻片（GNR@P（GMA-HEMA）载玻片）上形成三明治免疫测定来定量检测 7 种心血管生物标志物。该 AMMEFA 具有高特异性和灵敏度，线性范围跨越 5 个数量级，心肌肌钙蛋白 I（cTnI）、心脏型脂肪酸结合蛋白（H-FABP）、C 反应蛋白（CRP）、 copeptin、肌红蛋白、D-二聚体和 N 末端脑钠肽前体（NT-proBNP）的检测限（LOD）分别达到 0.07、0.2、65.7、0.6、0.2、8.3 和 0.3 pg mL。为了证明其实用性，该 AMMEFA 已应用于定量分析 140 份临床血浆样本中的 7 种心血管生物标志物。此外，通过最小绝对收缩和选择算子（LASSO）回归分析了心血管生物标志物的表达水平，健康供体（HD）、ACS 患者和 AAS 患者的接受者操作特征曲线（ROC）下面积（AUC）分别为 0.99、0.98 和 0.97。
