Wei Shihao, Ding Baolong, Wang Guiqin, Luo Shuangyan, Zhao Hongxi, Dan Xingang
College of Animal Science and Technology, Ningxia University, Yinchuan, China.
Front Microbiol. 2024 Apr 17;15:1364373. doi: 10.3389/fmicb.2024.1364373. eCollection 2024.
() is closely associated with the occurrence of puerperal metritis in dairy cows. carries some the virulence and multi-drug resistant genes, which pose a serious threat to the health of postpartum cows. In this study, was isolated and identified from the uterine contents of postpartum cows with puerperal metritis in the Ningxia region of China, and its phylogenetic subgroups were determined. Meanwhile, virulence and drug resistance genes carried by and drug sensitivity were detected, and the characteristics of virulence and drug resistance genes distribution in phylogroups were further analyzed. The results showed that the isolation rate of in puerperal metritis samples was 95.2%. was mainly divided into phylogroups B2 and D, followed by groups A and B1, and was more connected to O157:H7, O169:H4, and ECC-1470 type strains. The virulence genes were mainly dominated by (100%), (100%), (97%), (96%), (95%), (93.9%), and (93%), and the resistance genes were dominated by (99%), (71.7%), (66.7%), and (53.5%). Additionally, it was observed that the virulence and resistance gene phenotypes could be divided into two subgroups, with subgroup B2 and D having the highest distributions. Drug sensitivity tests also revealed that the was most sensitive to the fluoroquinolones enrofloxacin, followed by macrolides, aminoglycosides, tetracyclines, β-lactams, peptides and sulfonamides, and least sensitive to lincosamides. These results imply that pathogenic , which induces puerperal metritis of dairy cows in the Ningxia region of China, primarily belongs to the group B2 and D, contains multiple virulence and drug resistance genes, Moreover, has evolved resistance to several drugs including penicillin, lincomycin, cotrimoxazole, and streptomycin. It will offer specific guidelines reference for the prevention and treatment of puerperal metritis in dairy cows with infections in the Ningxia region of China.
（某病原体）与奶牛产后子宫内膜炎的发生密切相关。它携带一些毒力和多重耐药基因，对产后奶牛的健康构成严重威胁。在本研究中，从中国宁夏地区患有产后子宫内膜炎的奶牛子宫内容物中分离并鉴定了该病原体，并确定了其系统发育亚群。同时，检测了该病原体携带的毒力和耐药基因以及药敏情况，并进一步分析了毒力和耐药基因在该病原体系统发育群中的分布特征。结果表明，产后子宫内膜炎样本中该病原体的分离率为95.2%。该病原体主要分为B2和D系统发育群，其次是A群和B1群，且与O157:H7、O169:H4和ECC - 1470型菌株关系更为密切。毒力基因主要以（具体基因1）（100%）、（具体基因2）（100%）、（具体基因3）（97%）、（具体基因4）（96%）、（具体基因5）（95%）、（具体基因6）（93.9%）和（具体基因7）（93%）为主，耐药基因主要以（具体耐药基因1）（99%）、（具体耐药基因2）（71.7%）、（具体耐药基因3）（66.7%）和（具体耐药基因4）（53.5%）为主。此外，观察到毒力和耐药基因表型可分为两个亚群，其中B2和D亚群分布最高。药敏试验还表明，该病原体对氟喹诺酮类恩诺沙星最敏感，其次是大环内酯类、氨基糖苷类、四环素类、β - 内酰胺类、肽类和磺胺类，对林可酰胺类最不敏感。这些结果表明，在中国宁夏地区引起奶牛产后子宫内膜炎的致病性（该病原体）主要属于B2和D群，含有多个毒力和耐药基因，而且，该病原体已对包括青霉素、林可霉素、复方新诺明和链霉素在内的几种药物产生耐药性。这将为中国宁夏地区感染该病原体的奶牛产后子宫内膜炎的防治提供具体的指导参考。
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