Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt.
Department of Internal Medicine, Infectious and Fish Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt.
Theriogenology. 2022 Jul 1;186:108-113. doi: 10.1016/j.theriogenology.2022.03.019. Epub 2022 Apr 1.
The aim of this work is to study the expression profile of the vitamin D receptor (VDR), 1-α hydroxylase enzyme, and chemokine regulated on activation normal T-cell expressed and secreted genes (RANTES) genes in dairy cows with puerperal metritis, as well as to study the association between polymorphisms in the VDR gene and occurrence of such disease condition, which is considered a key to advances in the preventive medicine for such a problem in the future. Blood samples were collected from 60 dairy cows; from which 48 dairy cows proved to suffer puerperal metritis and other 12 apparently healthy recent parturient dairy cows were selected randomly for assessment the fold change variation in the expression profiles of the studied genes. Depending on the clinical examination, the normal cut-off point of the serum calcium, phosphorus, glucose, magnesium, potassium, sodium, and chloride, and the normal reference values of parathyroid hormone (PTH), 18 out of 48 dairy cows suffered puerperal metritis with a normal metabolic profile, which underwent further molecular analysis. In the studied dairy cows that suffered puerperal metritis with a normal metabolic profile, the expression pattern of the VDR and RANTES genes showed a significant up-regulation (P < 0.05), whereas the expression pattern of 1α-hydroxylase enzyme gene showed a significant down-regulation (P < 0.05) in comparison with those of healthy recent parturient dairy cows. The sequenced regions of the VDR gene (exon 7, partial intron 6, partial intron 7) didn't exhibit polymorphisms in their nucleotide sequence, and consecutively their amino acid sequence when compared to the published sequence on the gene bank. The results propose that the VDR gene (exon 7, partial intron 6, partial intron 7) polymorphisms do not play a role in the pathogenesis of puerperal metritis in dairy cows, excluding a role of the vitamin D in puerperal metritis.
本研究旨在研究患有产后子宫内膜炎的奶牛中维生素 D 受体 (VDR)、1-α羟化酶和趋化因子调节的正常 T 细胞表达和分泌基因 (RANTES) 基因的表达谱，并研究 VDR 基因多态性与该病发生的相关性，这被认为是未来预防此类疾病的关键。采集了 60 头奶牛的血液样本；其中 48 头奶牛被证实患有产后子宫内膜炎，另外 12 头近期分娩的健康奶牛被随机选择来评估所研究基因表达谱的变化倍数。根据临床检查，血清钙、磷、葡萄糖、镁、钾、钠和氯的正常截止值以及甲状旁腺激素 (PTH) 的正常参考值，48 头患有产后子宫内膜炎的奶牛中有 18 头代谢正常，进行了进一步的分子分析。在患有代谢正常的产后子宫内膜炎的奶牛中，VDR 和 RANTES 基因的表达模式表现出显著上调 (P < 0.05)，而 1α-羟化酶基因的表达模式则表现出显著下调 (P < 0.05)与健康近期分娩的奶牛相比。VDR 基因 (外显子 7、部分内含子 6、部分内含子 7) 的测序区域在核苷酸序列上没有多态性，与基因库中已发表的序列相比，其氨基酸序列也没有多态性。结果表明，VDR 基因 (外显子 7、部分内含子 6、部分内含子 7) 多态性在奶牛产后子宫内膜炎的发病机制中不起作用，排除了维生素 D 在产后子宫内膜炎中的作用。
