Universite de Pau Et Des Pays de L'Adour, E2S UPPA, CNRS, IPREM, Pau, France.
Novaptech, 2 Avenue Favard, 33170, Gradignan, France.
Anal Bioanal Chem. 2024 Jun;416(14):3295-3303. doi: 10.1007/s00216-024-05309-6. Epub 2024 May 2.
Thiabendazole, a widely used broad-spectrum fungicide in agriculture, poses risks to human health. To monitor its presence in water, we propose a fluorescent aptasensor utilizing Escherichia coli exonuclease I (Exo I). The findings demonstrate a linear correlation between thiabendazole concentrations and digestion percentage, with a detection limit (LOD) exceeding 1 µM and a determination coefficient (R) of 0.959. This aptamer-based fluorescence spectroscopy detection system holds promise for a rapid, specific, and sensitive analysis of thiabendazole in environmental waters and food matrices.
噻苯达唑是一种广泛应用于农业的广谱杀真菌剂，对人类健康构成威胁。为了监测其在水中的存在，我们提出了一种利用大肠杆菌核酸外切酶 I（Exo I）的荧光适配体传感器。研究结果表明，噻苯达唑浓度与消化百分比之间存在线性关系，检测限（LOD）超过 1 µM，决定系数（R）为 0.959。基于适配体的荧光光谱检测系统有望快速、特异性和灵敏地分析环境水样和食品基质中的噻苯达唑。
