用于眼表面手术的人羊膜生物合成替代品。

A Biosynthetic Alternative to Human Amniotic Membrane for Use in Ocular Surface Surgery.

机构信息

Academic Ophthalmology, Division of Clinical Neuroscience, School of Medicine, University of Nottingham, UK.

The Electrospinning Company, Oxfordshire, UK.

出版信息

Transl Vis Sci Technol. 2024 May 1;13(5):3. doi: 10.1167/tvst.13.5.3.

PURPOSE

The biosynthetic Symatix membrane (SM) was developed to replace fresh human amniotic membrane (hAM) in ocular surgical applications. The purpose of this study was to test the biocompatibility of the SM with human limbus-derived epithelial cells with regard to their physical and biological properties.

METHODS

Different physical properties of SM were tested ex vivo by simulation on human corneas. In vitro, primary limbal epithelial cells from limbal explants were used to test biological properties such as cell migration, proliferation, metabolic activity, and limbal epithelial cell markers on the SM, hAM, and freeze-dried amniotic membrane (FDAM).

RESULTS

The surgical handleability of the SM was equivalent to that of the hAM. Ultrastructural and histological studies demonstrated that epithelial cells on the SM had the typical tightly apposed, polygonal, corneal epithelial cell morphology. The epithelial cells were well stratified on the SM, unlike on the hAM and FDAM. Rapid wound healing occurred on the SM within 3 days. Immunofluorescence studies showed positive expression of CK-19, Col-1, laminin, ZO-1, FN, and p-63 on the SM, plastic, and FDAM compared to positive expression of ZO-1, Col-1, laminin, FN, and p63 and negative expression of CK-19 in the hAM.

CONCLUSIONS

These results indicate that the SM is a better substrate for limbal epithelial cell migration, proliferation, and tight junction formation. Altogether, the SM can provide a suitable alternative to the hAM for surgical application in sight-restoring operations.

TRANSLATIONAL RELEVANCE

The hAM, currently widely used in ocular surface surgery, has numerous variations and limitations. The biocompatibility of corneal epithelial cells with the SM demonstrated in this study suggests that it can be a viable substitute for the hAM.

目的

合成的 Symatix 膜（SM）旨在替代眼部手术中使用的新鲜人羊膜（hAM）。本研究的目的是测试 SM 与人角膜缘上皮细胞的生物相容性，评估其物理和生物学特性。

方法

通过在人角膜上进行模拟实验，测试 SM 的不同物理特性。体外，使用来自角膜缘组织片的原代角膜缘上皮细胞，测试 SM、hAM 和冻干羊膜（FDAM）上细胞迁移、增殖、代谢活性和角膜缘上皮细胞标志物等生物学特性。

结果

SM 的手术操作性与 hAM 相当。超微结构和组织学研究表明，SM 上的上皮细胞具有典型的紧密贴合、多角形、角膜上皮细胞形态。与 hAM 和 FDAM 不同，上皮细胞在 SM 上很好地分层。SM 上的伤口在 3 天内迅速愈合。免疫荧光研究显示，SM、塑料和 FDAM 上 CK-19、Col-1、层粘连蛋白、ZO-1、FN 和 p-63 的表达为阳性，而 hAM 上 ZO-1、Col-1、层粘连蛋白、FN 和 p63 的表达为阳性，CK-19 的表达为阴性。

结论

这些结果表明，SM 是角膜缘上皮细胞迁移、增殖和紧密连接形成的更好基质。总的来说，SM 可为视力恢复手术中的临床应用提供一种替代 hAM 的合适选择。

翻译感悟

句子 1：The biosynthetic Symatix membrane (SM) was developed to replace fresh human amniotic membrane (hAM) in ocular surgical applications.
译文：合成的 Symatix 膜（SM）旨在替代眼部手术中使用的新鲜人羊膜（hAM）。
解析：这里的 biosynthetic Symatix membrane (SM) 是指合成的 Symatix 膜，replaced fresh human amniotic membrane (hAM) 是指替代新鲜人羊膜（hAM），in ocular surgical applications 是指在眼部手术应用中。
句子 2：The epithelial cells were well stratified on the SM, unlike on the hAM and FDAM.
译文：与 hAM 和 FDAM 不同，上皮细胞在 SM 上很好地分层。
解析：这里的 well stratified 是指很好地分层，unlike on the hAM and FDAM 是指与 hAM 和 FDAM 不同。
句子 3：These results indicate that the SM is a better substrate for limbal epithelial cell migration, proliferation, and tight junction formation.
译文：这些结果表明，SM 是角膜缘上皮细胞迁移、增殖和紧密连接形成的更好基质。
解析：这里的 results indicate that 是指结果表明，a better substrate for limbal epithelial cell migration, proliferation, and tight junction formation 是指是角膜缘上皮细胞迁移、增殖和紧密连接形成的更好基质。