TEDA Institute of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin, 300457, China; Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, 23 Hongda Street, TEDA, Tianjin, 300457, China; Tianjin Key Laboratory of Microbial Functional Genomics, 23 Hongda Street, TEDA, Tianjin, 300457, China.
TEDA Institute of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin, 300457, China.
Anal Biochem. 2024 Aug;691:115553. doi: 10.1016/j.ab.2024.115553. Epub 2024 May 1.
We describe a microwave-assisted, methanol and acetic acid-free, inexpensive method for rapid staining of SDS-PAGE proteins. Only citric acid, benzoic acid, and Coomassie brilliant blue G-250 (CBG) were used. Microwave irradiation reduced the detection duration, and proteins in a clear background were visualized within 30 min of destaining, after 2 min of fixing and 12 min of staining. By using this protocol, comparable band intensities were obtained to the conventional methanol/acetic acid method.
我们描述了一种微波辅助的、无需甲醇和乙酸的、廉价的 SDS-PAGE 蛋白质快速染色方法。仅使用柠檬酸、苯甲酸和考马斯亮蓝 G-250（CBG）。微波辐射缩短了检测时间，在固定 2 分钟和染色 12 分钟后，只需 30 分钟即可在清晰的背景下观察到蛋白质，无需脱色。使用该方案，可获得与传统甲醇/乙酸方法相当的条带强度。
