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通过基于tANCHOR细胞的酶联免疫吸附测定法分析全细胞免疫产生的抗体。

Analysis of antibodies from whole-cell immunization by a tANCHOR cell-based ELISA.

作者信息

Bernauer Hubert, Schlör Anja, Maier Josef, Hanack Katja, Bannert Norbert, Ivanusic Daniel

机构信息

ATG:biosynthetics GmbH, Weberstraße 40, 79249 Merzhausen, Germany.

new/era/mabs GmbH, August-Bebel-Str. 89, 14482 Potsdam, Germany.

出版信息

MicroPubl Biol. 2024 Apr 17;2024. doi: 10.17912/micropub.biology.001201. eCollection 2024.

DOI:10.17912/micropub.biology.001201
PMID:38698909
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11063790/
Abstract

Monitoring specific antibodies derived from whole-cell immunization through cell-based ELISA methods poses challenges due to humoral responses against various cell proteins. In this report, we outline a technique involving pre-adsorption on cells to remove undesirable antibodies from immune serum. This step provides the subsequent monitoring of antibodies specific to the targeted antigen using a tANCHOR-based ELISA. Notably, this approach accelerates result acquisition, eliminating the necessity to purify the expressed antigen or obtain a customized peptide for coating assay plates.

摘要

通过基于细胞的ELISA方法监测全细胞免疫产生的特异性抗体面临挑战，因为存在针对各种细胞蛋白的体液反应。在本报告中，我们概述了一种技术，即对细胞进行预吸附，以从免疫血清中去除不需要的抗体。这一步骤使得随后能够使用基于tANCHOR的ELISA监测针对目标抗原的特异性抗体。值得注意的是，这种方法加快了结果获取速度，无需纯化表达的抗原或获取定制肽来包被检测板。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e63c/11063790/170c6636f08f/25789430-2024-micropub.biology.001201.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e63c/11063790/170c6636f08f/25789430-2024-micropub.biology.001201.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e63c/11063790/170c6636f08f/25789430-2024-micropub.biology.001201.jpg

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