Liu Qing, Cui Ruifei, Du Yuxin, Shen Junjie, Jin Cuili, Zhou Xiaojian
Marine Science and Technology Institute, College of Environmental Science and Engineering, Yangzhou University, Yangzhou, 225127, China.
Heliyon. 2024 Apr 16;10(9):e29641. doi: 10.1016/j.heliyon.2024.e29641. eCollection 2024 May 15.
In order to study the role of oil spills in the occurrence of green tide in the Yellow Sea, the physiological characteristics and photosynthetic activities of green tide causative-species was monitored under different conditions including two oil water-accommodated fractions (WAFs) of diesel oil and crude oil, dispersed water-accommodated fractions (DWAFs) and dispersant GM-2. The results showed that, the physiological parameters of including the growth, pigment, carbohydrate and protein contents decreased with the increased diesel oil WAF (WAF) concentration, while crude oil WAF (WAF) showed low concentration induction and high concentration inhibition effect. In addition, with the increase of WAFs concentration, two antioxidant activities were activated. However, compared with WAF alone and WAF alone, the mixture of oil and dispersant enhanced the toxicity on the above physiological characteristics of . On the other hand, the photosynthetic efficiency of showed a similar trend. Two WAFs showed significant concentration effects on the chlorophyll- fluorescence transients and JIP-test. The addition of dispersant further blocked the electron flow beyond QA and from plastoquinone (PQ) to PSI acceptor side, damaged the active OEC centers at the PSII donor side, suppressed the pool size and the reduction rate of PSI acceptor side, and reduced the energy transfer efficiency between PSII functional units. These results implied that the crude oil spills may induce the formation of green tide, and the oil dispersant GM-2 used after the oil spills is unlikely to further stimulate the scale of bloom, while the diesel oil spills is always not conducive to the outbreak of green tide of .
为研究溢油在黄海绿潮发生中的作用，在不同条件下监测了绿潮致病物种的生理特征和光合活性，这些条件包括柴油和原油的两种油-水可溶组分（WAFs）、分散的水可溶组分（DWAFs）以及分散剂GM-2。结果表明，随着柴油WAF（WAF）浓度的增加，包括生长、色素、碳水化合物和蛋白质含量在内的生理参数下降，而原油WAF（WAF）呈现低浓度诱导和高浓度抑制效应。此外，随着WAFs浓度的增加，两种抗氧化活性被激活。然而，与单独的WAF和单独的WAF相比，油与分散剂的混合物增强了对上述生理特征的毒性。另一方面，光合效率呈现类似趋势。两种WAFs对叶绿素荧光瞬变和JIP-测试显示出显著的浓度效应。分散剂的添加进一步阻断了QA之后以及从质体醌（PQ）到PSI受体侧的电子流，破坏了PSII供体侧的活性OEC中心，抑制了PSI受体侧的库大小和还原速率，并降低了PSII功能单元之间的能量转移效率。这些结果表明，原油泄漏可能诱发绿潮的形成，溢油后使用的油分散剂GM-2不太可能进一步刺激水华规模，而柴油泄漏始终不利于绿潮的爆发。
