Department of Food Science and Technology, Pukyong National University, 45 Yongso-ro Nam-gu, Busan, 48513, Republic of Korea.
Institute of Food Science, Pukyong National University, 45 Yongso-ro Nam-gu, Busan, 48513, Republic of Korea.
Mar Biotechnol (NY). 2024 Oct;26(5):876-890. doi: 10.1007/s10126-024-10321-z. Epub 2024 May 3.
Environmental pollution is a significant problem due to the improper disposal of plastics and shrimp shells outdoors. Therefore, the synthesis of biodegradable film from waste materials is highly important. The novelty of this research lies in the extraction of protein hydrolysates and chitosan from shrimp shells, as well as the fabrication of biodegradable film from these materials. In this study, the composite films were produced using the solution casting method. Moreover, the combined effect of ultrasound pretreatments (UPT) and natural deep eutectic solvents (NADES) was investigated as extraction media, to determine their potential impact on shrimp waste subcritical water hydrolysis (SWH). Shrimp shells were submitted to UPT in NADES solution, followed by SWH at different temperatures ranging from 150 to 230 °C under 3 MPa for 20 min. Then, the physiochemical properties and bioactivities of the hydrolysates were assessed to determine their suitability for use in biodegradable packaging films. Additionally, the physiochemical properties and bioactivities of the resulting hydrolysates were also analyzed. The highest amount of protein (391.96 ± 0.48 mg BSA/g) was obtained at 190 °C/UPT/NADES, and the average molecular size of the protein molecules was less than 1000 Da with different kinds of peptide. Overall, combined UPT and SWH treatments yielded higher antioxidant activity levels than individual treatments. Finally, the application of composite films was evaluated by wrapping fish samples and assessing their lipid oxidation. The use of higher concentrations of protein hydrolysates significantly delayed changes in the samples, thereby demonstrating the film's applicability.
由于户外对塑料和虾壳的不当处理，造成了环境污染这一严重问题。因此，利用废料合成可生物降解薄膜至关重要。本研究的新颖之处在于从虾壳中提取蛋白质水解物和壳聚糖，并利用这些材料制备可生物降解薄膜。在这项研究中，采用溶液浇铸法制备了复合薄膜。此外，还研究了超声预处理（UPT）和天然深共熔溶剂（NADES）联合作用作为提取介质，以确定其对虾废物亚临界水水解（SWH）的潜在影响。虾壳先在 NADES 溶液中进行 UPT，然后在 150 至 230°C 的不同温度下、3 MPa 下进行 SWH，时间为 20 分钟。随后，评估水解产物的物理化学性质和生物活性，以确定其在可生物降解包装薄膜中的适用性。此外，还分析了所得水解产物的物理化学性质和生物活性。在 190°C/UPT/NADES 条件下，蛋白质的提取量最高（391.96±0.48 mg BSA/g），且蛋白质分子的平均分子量小于 1000 Da，具有不同种类的肽。总的来说，联合 UPT 和 SWH 处理比单独处理产生更高的抗氧化活性水平。最后，通过包裹鱼样本来评估复合薄膜的应用，并评估其脂质氧化情况。使用更高浓度的蛋白质水解物显著延缓了样品的变化，从而证明了该薄膜的适用性。
