Lee D C, Rochford R, Todaro G J, Villarreal L P
Mol Cell Biol. 1985 Dec;5(12):3644-6. doi: 10.1128/mcb.5.12.3644-3646.1985.
Expression of the gene encoding transforming growth factor-alpha (TGF alpha) was examined in developing rat embryos by using a cloned TGF alpha cDNA as a hybridization probe. Northern blot analysis of RNA isolated from whole fetuses revealed that TGF alpha mRNA was present at relatively high levels in 8- through 10-day-old embryos and then declined to the low or undetectable level, which is characteristic of adult tissues before birth. The level of TGF alpha mRNA present during early gestation was similar to that present in retrovirus-transformed cells in culture, suggesting that TGF alpha expression is not highly localized in the embryo. These observations are consistent with the hypothesis that TGF alpha plays a role in development, possibly as a fetal growth factor.
利用克隆的转化生长因子α（TGFα）互补DNA作为杂交探针，检测了发育中的大鼠胚胎中编码TGFα的基因表达。对从整个胎儿分离的RNA进行的Northern印迹分析显示，TGFα信使核糖核酸（mRNA）在8至10日龄胚胎中以相对较高的水平存在，然后下降到低水平或无法检测到的水平，这是出生前成年组织的特征。妊娠早期存在的TGFα mRNA水平与培养中的逆转录病毒转化细胞中存在的水平相似，这表明TGFα表达在胚胎中并非高度局限。这些观察结果与TGFα在发育中起作用的假设一致，可能作为一种胎儿生长因子。
