Liu Ying, He Lei, Shi Jihong, Jiang Xia, Zhang Jianxin, Feng Yong, Liu Wei
Altern Ther Health Med. 2024 May 3.
To analyze the effect of Linc00511 on thyroid cancer through the miR-4739/RNF38 pathway.
A total of 78 patients in our hospital from July 2020 to July 2021 were collected, which were diagnosed with thyroid cancer after clinicopathological examination. Their cancer tissue samples were included in the thyroid cancer tissue group, and the fat 2 cm tissue samples were included in the para-cancer tissue group. Real-time quantitative PCR was used to detect the expression of Linc00511, miR-4739 and RNF38 in tissue samples from the two groups. Statistical analysis of data was performed using SPSS26.0. The correlation between Linc00511, miR-4739 and RNF38 were analyzed by Pearson's correlation coefficient. The expression of Linc00511 in thyroid cancer tissues with different clinicopathological characteristics were compared.
The expression levels of Linc00511 and RNF38 in thyroid cancer group were significantly higher than paracancer group, while miR-4739 levels were significantly lower (P < .05). Pearson correlation coefficient analysis showed that there was significant negative correlation between Linc00511 and miR-4739 expression and significant positive correlation between Linc00511 and RNF38 expression (P < .05). There was no significant difference in the expression of Linc00511 among different ages, sexes, and cancer types (P > .05). The expression of Linc00511 in patients with TNM stages I, II, and III were increased with the increase of TNM stage (P < .05). The expression of Linc00511 in patients with tumor diameter ≥1 cm was higher than that in patients with tumor diameter <1 cm and the difference was statistically significant (P < .05).
Linc00511 and RNF38 were significantly overexpressed in thyroid cancer tissues, while miR-4739 was significantly underexpressed. In thyroid cancer, Linc00511 can promote the invasion and metastasis of thyroid cancer cells by targeting miR-4739 and RNF38, and its expression level may reflect the progression of thyroid cancer, which can provide target reference for the clinical treatment of thyroid cancer.
通过miR - 4739/RNF38通路分析Linc00511对甲状腺癌的影响。
收集我院2020年7月至2021年7月共78例患者，经临床病理检查确诊为甲状腺癌。将其癌组织样本纳入甲状腺癌组织组，距癌灶2cm的脂肪组织样本纳入癌旁组织组。采用实时定量PCR检测两组组织样本中Linc00511、miR - 4739和RNF38的表达。使用SPSS26.0对数据进行统计分析。采用Pearson相关系数分析Linc00511、miR - 4739和RNF38之间的相关性。比较不同临床病理特征的甲状腺癌组织中Linc00511的表达。
甲状腺癌组中Linc00511和RNF38的表达水平显著高于癌旁组，而miR - 4739水平显著低于癌旁组（P < 0.05）。Pearson相关系数分析显示，Linc00511与miR - 4739表达呈显著负相关，Linc00511与RNF38表达呈显著正相关（P < 0.05）。不同年龄、性别和癌症类型的患者中Linc00511的表达无显著差异（P > 0.05）。TNM分期为I、II和III期的患者中，Linc00511的表达随TNM分期增加而升高（P < 0.05）。肿瘤直径≥1cm的患者中Linc00511的表达高于肿瘤直径<1cm的患者，差异具有统计学意义（P < 0.05）。
Linc00511和RNF38在甲状腺癌组织中显著过表达，而miR - 4739显著低表达。在甲状腺癌中，Linc00511可通过靶向miR - 4739和RNF38促进甲状腺癌细胞的侵袭和转移，其表达水平可能反映甲状腺癌的进展情况，可为甲状腺癌的临床治疗提供靶点参考。
