Wang Taoxia, Li Guiying, Xu Jinsheng, Bai Yaling, Liu Xiaoli, Jin Jingjing, Cheng Meijuan, Li Jianwei
Altern Ther Health Med. 2024 May 3.
The objective of this study was to investigate the relationship between vascular calcification, serum lncRNA H19, and Runt-Related Transcription Factor 2 mRNA expression in patients with uremia.
This study is a retrospective study which recruited 146 patients with uremia on dialysis from December 2021 to November 2022. Participants were divided into the VC and non-VC groups based on their chest X-ray calcification ratings. General and clinical data were collected from all patients. Serum H19, Runx2 mRNA, mineral bone disease effectors, and other blood markers were tested. Univariate analysis was performed to compare the changes in each clinical index between these two groups of patients. A multi-factor logistic regression analysis of risk factors for VC was performed. Receiver operating characteristics analyzed the H19 and Runx2 for their diagnostic values for VC. Pearson's test was used to analyze the correlation between the H19 and Runx2 expression and the factors influencing VC.
Patients in the VC group had significantly higher creatinine, serum phosphorus, calcium, BMP-2, FGF-23, OPG, and iPTH levels than those in the non-VC group (P < .05), while their albumin levels were significantly lower than those in the non-VC group (P < .05). The expression of H19 and Runx2 mRNA was significantly upregulated in the serum of VC patients (P < .05). H19 was significantly positively correlated with creatinine, serum phosphorus, calcium, BMP-2, OPG, and iPTH (P < .05). Runx2 mRNA was significantly positively correlated with creatinine, FGF-23, and iPTH (P < .05 ), while there was no significant correlation with other factors(P > .05). Albumin, BMP-2, iPTH, H19, and Runx2 were independent correlative-factors of uremic VC. In addition, the combined H19 and Runx2 test (AUC=0.850; 95% CI: 0.781-0.903) had good diagnostic values for the development of VC.
Serum H19 and Runx2 levels are significantly associated with VC-related factors and are independent risk factors for uremic VC, and their levels contribute to the diagnosis of uremic VC.
本研究旨在探讨尿毒症患者血管钙化、血清长链非编码RNA H19与 runt 相关转录因子 2 mRNA 表达之间的关系。
本研究为回顾性研究，纳入了 2021 年 12 月至 2022 年 11 月期间 146 例接受透析的尿毒症患者。根据胸部 X 线钙化分级将参与者分为血管钙化（VC）组和非 VC 组。收集所有患者的一般资料和临床数据。检测血清 H19、Runx2 mRNA、矿物质骨病效应因子及其他血液标志物。进行单因素分析以比较两组患者各临床指标的变化。对 VC 的危险因素进行多因素逻辑回归分析。采用受试者工作特征曲线分析 H19 和 Runx2 对 VC 的诊断价值。采用 Pearson 检验分析 H19 和 Runx2 表达与影响 VC 的因素之间的相关性。
VC 组患者的肌酐、血清磷、钙、骨形态发生蛋白-2（BMP-2）、成纤维细胞生长因子-23（FGF-23）、骨保护素（OPG）和全段甲状旁腺激素（iPTH）水平显著高于非 VC 组（P < 0.05），而其白蛋白水平显著低于非 VC 组（P < 0.05）。VC 患者血清中 H19 和 Runx2 mRNA 的表达显著上调（P < 0.05）。H19 与肌酐、血清磷、钙、BMP-2、OPG 和 iPTH 显著正相关（P < 0.05）。Runx2 mRNA 与肌酐、FGF-23 和 iPTH 显著正相关（P < 0.05），而与其他因素无显著相关性（P > 0.05）。白蛋白、BMP-2、iPTH、H19 和 Runx2 是尿毒症性 VC 的独立相关因素。此外，联合检测 H19 和 Runx2（AUC = 0.850；95%CI：0.781 - 0.903）对 VC 的发生具有良好的诊断价值。
血清 H19 和 Runx2 水平与 VC 相关因素显著相关，是尿毒症性 VC 的独立危险因素，其水平有助于尿毒症性 VC 的诊断。
