Department of Chemistry and Biochemistry, University of Delaware, 590 Avenue 1743, Newark, DE, 19713, USA.
Top Curr Chem (Cham). 2024 May 4;382(2):15. doi: 10.1007/s41061-024-00455-y.
Aligned with the increasing importance of bioorthogonal chemistry has been an increasing demand for more potent, affordable, multifunctional, and programmable bioorthogonal reagents. More advanced synthetic chemistry techniques, including transition-metal-catalyzed cross-coupling reactions, C-H activation, photoinduced chemistry, and continuous flow chemistry, have been employed in synthesizing novel bioorthogonal reagents for universal purposes. We discuss herein recent developments regarding the synthesis of popular bioorthogonal reagents, with a focus on s-tetrazines, 1,2,4-triazines, trans-cyclooctenes, cyclooctynes, hetero-cycloheptynes, and -trans-cycloheptenes. This review aims to summarize and discuss the most representative synthetic approaches of these reagents and their derivatives that are useful in bioorthogonal chemistry. The preparation of these molecules and their derivatives utilizes both classical approaches as well as the latest organic chemistry methodologies.
与生物正交化学的重要性不断提高相一致的是，人们对更有效、更经济、多功能和可编程的生物正交试剂的需求不断增加。更先进的合成化学技术，包括过渡金属催化交叉偶联反应、C-H 活化、光诱导化学和连续流动化学，已被用于合成用于通用目的的新型生物正交试剂。本文讨论了关于流行的生物正交试剂的合成的最新进展，重点是 s-四嗪、1,2,4-三嗪、反式环辛烯、环辛炔、杂环庚炔和 -反式环庚烯。本综述旨在总结和讨论这些试剂及其在生物正交化学中有用的衍生物的最具代表性的合成方法。这些分子及其衍生物的制备既利用了经典方法，也利用了最新的有机化学方法。
