São Paulo State University (UNESP), School of Pharmaceutical Sciences, Department of Clinical Analysis, Araraquara, São Paulo, Brazil.
São Paulo State University (UNESP), School of Dentistry, Department of Dental Materials and Prosthodontics, Araraquara, São Paulo, Brazil.
J Photochem Photobiol B. 2024 Jun;255:112905. doi: 10.1016/j.jphotobiol.2024.112905. Epub 2024 Apr 23.
Bacterial antibiotic resistance is one of the most significant challenges for public health. The increase in bacterial resistance, mainly due to microorganisms harmful to health, and the need to search for alternative treatments to contain infections that cannot be treated by conventional antibiotic therapy has been aroused. An alternative widely studied in recent decades is antimicrobial photodynamic therapy (aPDT), a treatment that can eliminate microorganisms through oxidative stress. Although this therapy has shown satisfactory results in infection control, it is still controversial in the scientific community whether bacteria manage to develop resistance after successive applications of aPDT. Thus, this work provides an overview of the articles that performed successive aPDT applications in models using bacteria published since 2010, focusing on sublethal dose cycles, highlighting the main PSs tested, and addressing the possible mechanisms for developing tolerance or resistance to aPDT, such as efflux pumps, biofilm formation, OxyR and SoxRS systems, catalase and superoxide dismutase enzymes and quorum sensing.
细菌抗生素耐药性是公共卫生面临的最大挑战之一。由于对健康有害的微生物，细菌耐药性的增加，以及需要寻找替代治疗方法来控制常规抗生素治疗无效的感染，这一问题已经引起了关注。近年来广泛研究的一种替代方法是抗菌光动力疗法（aPDT），这种治疗方法可以通过氧化应激来消除微生物。尽管这种疗法在控制感染方面显示出了令人满意的效果，但科学界仍存在争议，即细菌在连续应用 aPDT 后是否能够产生耐药性。因此，这项工作概述了自 2010 年以来发表的使用细菌的模型中进行连续 aPDT 应用的文章，重点介绍了亚致死剂量循环，突出了测试的主要 PS，并讨论了对 aPDT 产生耐受性或耐药性的可能机制，如外排泵、生物膜形成、OxyR 和 SoxRS 系统、过氧化氢酶和超氧化物歧化酶以及群体感应。
