Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou, China; Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding and Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, China; LiveGene-Centre for Tropical Livestock Genetics and Health (CTLGH), International Livestock Research Institute (ILRI), Addis Ababa, Ethiopia.
Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou, China; Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding and Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, China.
Poult Sci. 2024 Jun;103(6):103716. doi: 10.1016/j.psj.2024.103716. Epub 2024 Apr 3.
Coccidiosis, a protozoan disease that substantially impacts poultry production, is characterized by an intracellular parasite. The study utilized 48 one-day-old Horro chickens, randomly divided into the infected (I) and control (C) groups. The challenge group of chickens were administered Eimeria maxima oocysts via oral gavage at 21-days-old, and each chicken received 2 mL containing 7×10 sporulated oocysts. The total RNAs of chicken jejunum and cecum tissues were isolated from three samples, each from I and C groups. Our study aimed to understand the host immune-parasite interactions and compare immune response mRNA profiles in chicken jejunum and cecum tissues at 4 and 7 days postinfection with Eimeria maxima. The results showed that 823 up- and 737 down-regulated differentially expressed mRNAs (DEmRNAs) in jejunum at 4 d infection and control (J4I vs. J4C), and 710 up- and 368 down-regulated DEmRNAs in jejunum at 7 days infection and control (J7I vs. J7C) were identified. In addition, DEmRNAs in cecum tissue, 1424 up- and 1930 down-regulated genes in cecum at 4 days infection and control (C4I vs. C4C), and 77 up- and 191 down-regulated genes in cecum at 7 days infection and control (C7I vs. C7C) were detected. The crucial DEmRNAs, including SLC7A5, IL1R2, GLDC, ITGB6, ADAMTS4, IL1RAP, TNFRSF11B, IMPG2, WNT9A, and FOXF1, played pivotal roles in the immune response during Eimeria maxima infection of chicken jejunum. In addition, the potential detection of FSTL3, RBP7, CCL20, DPP4, PRKG2, TFPI2, and CDKN1A in the cecum during the host immune response against Eimeria maxima infection is particularly noteworthy. Furthermore, our functional enrichment analysis revealed the primary involvement of DEmRNAs in small molecule metabolic process, immune response function, inflammatory response, and toll-like receptor 10 signaling pathway in the jejunum at 4 and 7 days postinfection. Similarly, in the cecum, DEmRNAs at 4 and 7 days postinfection were enriched in processes related to oxidative stress response and immune responses. Our findings provide new insights and contribute significantly to the field of poultry production and parasitology.
球虫病是一种严重影响家禽生产的原生动物疾病，其特征为细胞内寄生虫。本研究使用了 48 只 1 日龄的 Horro 鸡，随机分为感染组（I）和对照组（C）。在 21 日龄时，通过口服灌胃向感染组鸡群施用柔嫩艾美耳球虫卵囊，每只鸡接受含有 7×10 个孢子化卵囊的 2 mL 溶液。从每组（I 和 C）的三个样本中分离出鸡空肠和盲肠组织的总 RNA。我们的研究旨在了解宿主免疫-寄生虫相互作用，并比较柔嫩艾美耳球虫感染鸡空肠和盲肠组织在 4 天和 7 天后的免疫反应 mRNA 谱。结果表明，在 4 天感染和对照组（J4I 与 J4C）中，空肠中有 823 个上调和 737 个下调的差异表达 mRNA（DEmRNAs），在 7 天感染和对照组（J7I 与 J7C）中，空肠中有 710 个上调和 368 个下调的 DEmRNAs。此外，在盲肠组织中，在 4 天感染和对照组（C4I 与 C4C）中有 1424 个上调和 1930 个下调的 DEmRNAs，在 7 天感染和对照组（C7I 与 C7C）中有 77 个上调和 191 个下调的 DEmRNAs。在柔嫩艾美耳球虫感染鸡空肠的免疫反应中，包括 SLC7A5、IL1R2、GLDC、ITGB6、ADAMTS4、IL1RAP、TNFRSF11B、IMPG2、WNT9A 和 FOXF1 在内的关键 DEmRNAs 发挥了重要作用。此外，在宿主对柔嫩艾美耳球虫感染的免疫反应中，在盲肠中检测到 FSTL3、RBP7、CCL20、DPP4、PRKG2、TFPI2 和 CDKN1A 的潜在表达也值得关注。此外，我们的功能富集分析表明，在感染后 4 天和 7 天的空肠中，DEmRNAs 主要参与小分子代谢过程、免疫反应功能、炎症反应和 Toll 样受体 10 信号通路。同样，在盲肠中，在感染后 4 天和 7 天的 DEmRNAs 中富集了与氧化应激反应和免疫反应相关的过程。我们的研究结果提供了新的见解，并为家禽生产和寄生虫学领域做出了重要贡献。
