Hadawale Sakshi, Sharma Sanjay
Department of Quality Assurance, Shobhaben Pratapbhai Patel School of Pharmacy & Technology Management, SVKM's NMIMS, Deemed to be University, Vile Parle West, Mumbai, Maharashtra, 400056, India.
Curr Diabetes Rev. 2025;21(5):e030524229629. doi: 10.2174/0115733998288292240409060854.
DPP-4 inhibitors, or gliptins, are new oral antidiabetic drugs for type 2 diabetes. They help to regulate insulin and glucagon. These drugs have the advantage of a lower risk of hypoglycemia compared to some other diabetes medications and are typically prescribed when metformin and sulphonylureas have become less effective.
This review analyses a range of analytical and bioanalytical methods for DPP-4 inhibitors, that use spectroscopic techniques, chromatographic, and hyphenated techniques for analysis. So far, no review comprising all DPP-4 inhibitors has been presented. The primary objective of this review is to present the analysts with various analytical and bioanalytical methods for the quantification and estimation of DPP-4 inhibitors in different matrices.
To improve understanding, a review was carried out by creating a database of pre-existing research from digital sources such as ScienceDirect, and PubMed. The methodology is shown in the flowchart of the literature selection process.
The comprehensive assessment of methods for analysing DPP-4 inhibitors can be a valuable resource for researchers and healthcare practitioners. Hitherto, no review encompassing all DPP-4 inhibitors has been presented. Therefore, gaps in the data available on a particular subject, need to be required to collect data on a particular construct. The review suggests that chromatographic techniques were majorly used for analysis wherein solvents like acetonitrile, methanol, and buffer solutions were used as mobile phases that can deteriorate HPLC columns and equipment. So, scientists could investigate new methods for the assessment of DPP-4 inhibitors using more eco-friendly solvents.
二肽基肽酶-4（DPP-4）抑制剂，即格列汀类药物，是用于治疗2型糖尿病的新型口服抗糖尿病药物。它们有助于调节胰岛素和胰高血糖素。与其他一些糖尿病药物相比，这些药物具有低血糖风险较低的优势，通常在二甲双胍和磺脲类药物疗效降低时使用。
本综述分析了一系列用于DPP-4抑制剂的分析和生物分析方法，这些方法使用光谱技术、色谱技术和联用技术进行分析。到目前为止，尚未有涵盖所有DPP-4抑制剂的综述。本综述的主要目的是向分析人员介绍用于定量和估算不同基质中DPP-4抑制剂的各种分析和生物分析方法。
为了增进理解，通过创建一个来自ScienceDirect和PubMed等数字资源的现有研究数据库进行了综述。该方法在文献选择过程的流程图中展示。
对DPP-4抑制剂分析方法的全面评估对研究人员和医疗从业者而言可能是一份宝贵的资源。迄今为止，尚未有涵盖所有DPP-4抑制剂的综述。因此，需要填补特定主题现有数据中的空白，收集关于特定结构的数据。该综述表明，色谱技术主要用于分析，其中乙腈、甲醇和缓冲溶液等溶剂用作流动相，这可能会使高效液相色谱（HPLC）柱和设备性能下降。所以，科学家们可以研究使用更环保溶剂评估DPP-4抑制剂的新方法。
