González F C, Fantuzzi L, Sanahuja M C
Cátedra de Microbiología Agrícola, Facultad de Agronomía, Universidad de Buenos Aires, Argentina.
Rev Argent Microbiol. 1985;17(4):225-8.
Prill and Hammer's method (4) for microdetermination of diacetyl was modified by several authors (1-3, 7), but retaining the same principle: diacetyl is converted into dimethylglyoxime by reaction with hydroxylamine; the oxime is subsequently converted into a pink ammonoferrous glyoximate and its colour is measured by absorbance at 530 nm. In the present communication a procedure based on the method of Pack et al. (3) is proposed with the following modifications: 1. Omission of carboy and suction flask; 2. Elongation of the connecting tubing between sample and trap tubes and relocation of the trap tubes to a higher level. 3. Replacement of rubber tubing by pvc connection and of rubber stoppers by neoprene ones. 4. A more accurate regulation of the nitrogen flow. 5. Protection of the Fe SO4 against oxidation. 6. Omission of the rinse of the trap tips with K2 HPO4 solution. 7. Use of diacetyl as a standard instead of dimethylglyoxime.
普里尔和哈默微量测定双乙酰的方法（4）被几位作者（1 - 3，7）改进过，但仍保留相同原理：双乙酰通过与羟胺反应转化为二甲基乙二肟；随后肟转化为粉红色的氨基亚铁乙二肟酸，通过在530nm处的吸光度测量其颜色。在本通讯中，提出了一种基于帕克等人（3）方法的程序，并做了以下修改：1. 省略大玻璃瓶和吸滤瓶；2. 延长样品管与捕集管之间的连接管，并将捕集管移至更高位置；3. 用聚氯乙烯连接件代替橡胶管，用氯丁橡胶塞代替橡胶塞；4. 更精确地调节氮气流；5. 保护硫酸亚铁不被氧化；6. 省略用磷酸氢二钾溶液冲洗捕集管尖端；7. 使用双乙酰作为标准品而非二甲基乙二肟。
