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通过Opal/TSA多重免疫荧光法检测基质B淋巴细胞对三阴性乳腺癌预后的影响

Stromal B Lymphocytes Affecting Prognosis in Triple-Negative Breast Cancer by Opal/TSA Multiplexed Immunofluorescence.

作者信息

Fang Min, Yin Wei, Qiu Chunyan, Song Tao, Lin Baihua, Wang Ying, Xiong Hanchu, Wu Shixiu

机构信息

Department of Radiation Oncology, The Affiliated Hangzhou Hospital of Nanjing Medical University, Hangzhou, Zhejiang, People's Republic of China.

Cancer Center, Department of Radiation Oncology, Zhejiang Provincial People's Hospital(Affiliated People's Hospital), Hangzhou Medical College, Hangzhou, Zhejiang, People's Republic of China.

出版信息

Int J Womens Health. 2024 Apr 29;16:755-767. doi: 10.2147/IJWH.S444202. eCollection 2024.

DOI:10.2147/IJWH.S444202
PMID:38706691
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11067943/
Abstract

OBJECTIVE

Immune cells play a key role in tumor microenvironment. The purpose of this study was to investigate the infiltration and clinical indication of immune cells including their combined prognostic value in microenvironment of triple negative breast cancer.

METHODS

We investigated 100 patients with triple negative breast cancer by Opal/Tyramide Signal Amplification multispectral immunofluorescence between 2003 and 2017 at Zhejiang Provincial people's Hospital. Intratumoral and stromal immune cells of triple negative breast cancer were classified and quantitatively analyzed. Survival outcomes were compared using the Kaplan-Meier method and further analyzed with multivariate analysis.

RESULTS

Infiltration level of stromal B lymphocytes, stromal and intratumoral CD8+ T cells, stromal CD4+ T cells, stromal PD-L1 and intratumoral tumor associated macrophages 2 cells were shown as independent factors affecting disease-free survival and overall survival in univariate analysis. Stromal B lymphocytes, T stage, N stage and pathological type were independent predictive factors for both DFS and OS in multivariate analysis. We firstly found that patients with B lymphocytes-enriched subtypes have a better prognosis than those with T lymphocytes-enriched subtypes and tumor-associated macrophage-enriched subtypes.

CONCLUSION

The present study identified a bunch of immune targets and subtypes, which could be exploited in future combined immunotherapy/chemotherapy strategies for triple negative breast cancer patients.

摘要

目的

免疫细胞在肿瘤微环境中起关键作用。本研究旨在探讨免疫细胞在三阴性乳腺癌微环境中的浸润情况、临床指标及其联合预后价值。

方法

2003年至2017年期间，我们在浙江省人民医院采用Opal/酪胺信号放大多光谱免疫荧光法对100例三阴性乳腺癌患者进行了研究。对三阴性乳腺癌的瘤内和基质免疫细胞进行分类并定量分析。采用Kaplan-Meier法比较生存结果，并进一步进行多因素分析。

结果

在单因素分析中，基质B淋巴细胞、基质和瘤内CD8+ T细胞、基质CD4+ T细胞、基质PD-L1和瘤内肿瘤相关巨噬细胞2细胞的浸润水平显示为影响无病生存期和总生存期的独立因素。在多因素分析中，基质B淋巴细胞、T分期、N分期和病理类型是DFS和OS的独立预测因素。我们首次发现，富含B淋巴细胞亚型的患者比富含T淋巴细胞亚型和肿瘤相关巨噬细胞亚型的患者预后更好。

结论

本研究确定了一系列免疫靶点和亚型，可用于未来三阴性乳腺癌患者的联合免疫治疗/化疗策略。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23d8/11067943/a9ad5e380c35/IJWH-16-755-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23d8/11067943/64eaa4dfd0b9/IJWH-16-755-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23d8/11067943/e9546b37aae0/IJWH-16-755-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23d8/11067943/3c839fb74bfe/IJWH-16-755-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23d8/11067943/a9ad5e380c35/IJWH-16-755-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23d8/11067943/64eaa4dfd0b9/IJWH-16-755-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23d8/11067943/e9546b37aae0/IJWH-16-755-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23d8/11067943/3c839fb74bfe/IJWH-16-755-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23d8/11067943/a9ad5e380c35/IJWH-16-755-g0004.jpg

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