Yang Shu, Hou Qinghua, Li Nan, Wang Pengbin, Zhao Huaxian, Chen Qingxiang, Qin Xinyi, Huang Jiongqing, Li Xiaoli, Liao Nengjian, Jiang Gonglingxia, Dong Ke, Zhang Tianyu
Key Laboratory of Climate, Resources and Environment in Continental Shelf Sea and Deep Sea of Department of Education of Guangdong Province, Department of Oceanography, Key Laboratory for Coastal Ocean Variation and Disaster Prediction, College of Ocean and Meteorology, Guangdong Ocean University, Zhanjiang, China.
Key Laboratory of Environment Change and Resources Use in Beibu Gulf, Ministry of Education (Nanning Normal University), Nanning, China.
Front Microbiol. 2024 Apr 19;15:1365546. doi: 10.3389/fmicb.2024.1365546. eCollection 2024.
Microorganisms, especially rare microbial species, are crucial in estuarine ecosystems for driving biogeochemical processes and preserving biodiversity. However, the understanding of the links between ecosystem multifunctionality (EMF) and the diversity of rare bacterial taxa in estuary ecosystems remains limited. Employing high-throughput sequencing and a variety of statistical methods, we assessed the diversities and assembly process of abundant and rare bacterioplankton and their contributions to EMF in a subtropical estuary. Taxonomic analysis revealed Proteobacteria as the predominant phylum among both abundant and rare bacterial taxa. Notably, rare taxa demonstrated significantly higher taxonomic diversity and a larger species pool than abundant taxa. Additionally, our findings highlighted that deterministic assembly processes predominantly shape microbial communities, with heterogeneous selection exerting a stronger influence on rare taxa. Further analysis reveals that rare bacterial beta-diversity significantly impacts to EMF, whereas alpha diversity did not. The partial least squares path modeling (PLS-PM) analysis demonstrated that the beta diversity of abundant and rare taxa, as the main biotic factor, directly affected EMF, while temperature and total organic carbon (TOC) were additional key factors to determine the relationship between beta diversity and EMF. These findings advance our understanding of the distribution features and ecological knowledge of the abundant and rare taxa in EMF in subtropical estuaries, and provide a reference for exploring the multifunctionality of different biospheres in aquatic environments.
微生物，尤其是稀有微生物物种，在河口生态系统中对于推动生物地球化学过程和保护生物多样性至关重要。然而，对于河口生态系统中生态系统多功能性（EMF）与稀有细菌类群多样性之间联系的理解仍然有限。我们采用高通量测序和多种统计方法，评估了亚热带河口丰富和稀有浮游细菌的多样性、组装过程及其对EMF的贡献。分类学分析表明，变形菌门是丰富和稀有细菌类群中占主导地位的门类。值得注意的是，稀有类群的分类学多样性显著高于丰富类群，且物种库更大。此外，我们的研究结果强调，确定性组装过程主要塑造微生物群落，异质性选择对稀有类群的影响更强。进一步分析表明，稀有细菌的β多样性对EMF有显著影响，而α多样性则没有。偏最小二乘路径建模（PLS-PM）分析表明，丰富和稀有类群的β多样性作为主要生物因子直接影响EMF，而温度和总有机碳（TOC）是决定β多样性与EMF之间关系的其他关键因素。这些发现推进了我们对亚热带河口EMF中丰富和稀有类群分布特征及生态知识的理解，并为探索水生环境中不同生物圈的多功能性提供了参考。
