血浆衍生的细胞外囊泡可改善胚胎发育。
Plasma-derived extracellular vesicles improve mice embryo development.
机构信息
Department of Anatomical Sciences, Faculty of Medicine, Zanjan University of Medical Sciences (ZUMS), Zanjan, Iran.
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
出版信息
Mol Biol Rep. 2024 May 6;51(1):621. doi: 10.1007/s11033-024-09500-x.
BACKGROUND
To investigate the effect of plasma-derived extracellular vesicles (EVs) or conventional medium in fertilization and early embryo development rate in mice.
METHODS AND RESULTS
MII oocytes (matured in vivo or in vitro conditions) were obtained from female mice. The extracellular vesicles were isolated by ultracentrifugation of plasma and were analyzed and measured for size and morphology by dynamic light scattering (DLS) and transmission electron microscopy (TEM). By western blotting analysis, the EVs proteins markers such as CD82 protein and heat shock protein 90 (HSP90) were investigated. Incorporating DiI-labeled EVs within the oocyte cytoplasm was visible at 23 h in oocyte cytoplasm. Also, the effective proteins in the early reproductive process were determined in isolated EVs by western blotting. These EVs had a positive effect on the fertilization rate (P < 0.05). The early embryo development (8 cell, morula and blastocyst stages) was higher in groups supplemented with EVs (P < 0.01).
CONCLUSION
Our findings showed that supplementing in vitro maturation media with EVs derived- plasma was beneficial for mice's embryo development.
背景
研究了血浆衍生的细胞外囊泡（EVs）或常规培养基对小鼠受精和早期胚胎发育率的影响。
方法和结果
从雌性小鼠中获得 MII 卵母细胞（体内或体外成熟）。通过超速离心从血浆中分离细胞外囊泡，并通过动态光散射（DLS）和透射电子显微镜（TEM）分析和测量其大小和形态。通过 Western blot 分析，研究了 EVs 蛋白标志物，如 CD82 蛋白和热休克蛋白 90（HSP90）。在卵母细胞质内可见 DiI 标记的 EVs 在 23 小时内进入卵母细胞质。此外，通过 Western blot 确定了分离的 EVs 中早期生殖过程中的有效蛋白。这些 EVs 对受精率有积极影响（P<0.05）。补充 EVs 的组的早期胚胎发育（8 细胞、桑椹胚和囊胚阶段）更高（P<0.01）。
结论
我们的研究结果表明，在体外成熟培养基中添加源自血浆的 EVs 有利于小鼠胚胎的发育。
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