[固定化青霉素酰胺酶催化苯乙酰胺水解的动力学]
[Kinetics of phenylacetamide hydrolysis by immobilized penicillinamidase].
作者信息
Milman I A, Geĭman I I, Slavinskaia V A
出版信息
Biokhimiia. 1979 Aug;44(8):1377-80.
PMID:387097
Abstract
The kinetics of phenylacetamide hydrolysis catalyzed by polyacrylamide gel-immobilized penicillinamidase were studied. The Km and Kp values obtained were compared to the literary data for the specific substrate--benzylpenicillin. It was shown that the type of inhibition by the reaction product was the same, whereas the efficiency of binding of phenylacetic acid depended on the substrate structure.
摘要
研究了聚丙烯酰胺凝胶固定化青霉素酰胺酶催化苯乙酰胺水解的动力学。将获得的Km和Kp值与特定底物苄青霉素的文献数据进行了比较。结果表明，反应产物的抑制类型相同，而苯乙酸的结合效率取决于底物结构。
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[Kinetics of phenylacetamide hydrolysis by immobilized penicillinamidase].[固定化青霉素酰胺酶催化苯乙酰胺水解的动力学]
Biokhimiia. 1979 Aug;44(8):1377-80.
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