Wuhan Fisheries Science Research Institute, Wuhan Academy of Agricultural Sciences, Wuhan, Hubei Province, 430070, China; College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei Province, 430070, China.
State Key Laboratory of Freshwater Ecology and Biotechnology, and Key Laboratory of Aquaculture Disease Control, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, 430072, China.
Fish Shellfish Immunol. 2024 Jun;149:109614. doi: 10.1016/j.fsi.2024.109614. Epub 2024 May 6.
Chemokines are critical molecules involved in immune reaction and immune system homeostasis, and some chemokines play a role in antiviral immunity. It is not known if the C-C motif chemokine ligand 3 (CCL3), a member of the CC chemokine family, possesses antiviral properties in fish. In this study, a ccl3 was cloned from the mandarin fish (Siniperca chuatsi), and it has an open reading frame (ORF) of 276 base pairs, which are predicted to encode a 91-amino acid peptide. Mandarin fish CCL3 revealed conserved sequence features with four cysteine residues and closely relationships with the CCL3s from other vertebrates based on the sequence alignment and phylogenetic analysis. The transcripts of ccl3 were notably enriched in immune-related organs, such as spleen and gills in healthy mandarin fish, and the ccl3 was induced in the isolated mandarin fish brain (MFB) cells following infection with infectious spleen and kidney necrosis virus (ISKNV). Moreover, in MFB cells, overexpression of CCL3 induced immune factors, such as IL1β, TNFα, MX, IRF1 and IFNh, and exhibited antiviral activity against ISKNV. This study sheds light on the immune role of CCL3 in immune response of mandarin fish, and its antiviral defense mechanism is of interest for further investigation.
趋化因子是参与免疫反应和免疫系统稳态的关键分子，一些趋化因子在抗病毒免疫中发挥作用。目前尚不清楚 C-C 基序趋化因子配体 3（CCL3）是否具有鱼类的抗病毒特性。在本研究中，从鳜鱼（Siniperca chuatsi）中克隆了一个 ccl3，其开放阅读框（ORF）为 276 个碱基对，预测编码 91 个氨基酸的肽。根据序列比对和系统发育分析，鳜鱼 CCL3 具有保守的序列特征，包括四个半胱氨酸残基，与其他脊椎动物的 CCL3 密切相关。在健康鳜鱼的免疫相关器官中，如脾脏和鳃中，ccl3 的转录本明显富集，在感染传染性脾坏死病毒（ISKNV）后，分离的鳜鱼脑（MFB）细胞中 ccl3 被诱导。此外，在 MFB 细胞中，CCL3 的过表达诱导了免疫因子，如 IL1β、TNFα、MX、IRF1 和 IFNh，并表现出对 ISKNV 的抗病毒活性。本研究揭示了 CCL3 在鳜鱼免疫反应中的免疫作用，其抗病毒防御机制值得进一步研究。
