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嗜麦芽窄食单胞菌USTB-05中MlrB催化线性化微囊藻毒素的酶促机制。

Enzymatic mechanism of MlrB for catalyzing linearized microcystins by sp. USTB-05.

作者信息

Teng Junhui, Xu Qianqian, Zhang Haiyang, Yu Ruipeng, Liu Chao, Song Meijie, Cao Xiaoyu, Du Xinyue, Tao Suxuan, Yan Hai

机构信息

School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, China.

Beijing Institute for Drug Control, Beijing, China.

出版信息

Front Microbiol. 2024 Apr 22;15:1389235. doi: 10.3389/fmicb.2024.1389235. eCollection 2024.

DOI:10.3389/fmicb.2024.1389235
PMID:38711965
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11070527/
Abstract

Microcystins (MCs) are the most widespread cyanobacterial toxins in eutrophic water body. As high toxic intermediate metabolites, linearized MCs are further catalyzed by linearized microcystinase (MlrB) of sp. USTB-05. Here MlrB structure was studied by comprizing with a model representative of the penicillin-recognizing enzyme family via homology modeling. The key active sites of MlrB were predicted by molecular docking, and further verified by site-directed mutagenesis. A comprehensive enzymatic mechanism for linearized MCs biodegradation by MlrB was proposed: S77 transferred a proton to H307 to promote a nucleophilic attack on the peptide bond (Ala-Leu in MC-LR or Ala-Arg in MC-RR) of linearized MCs to form the amide intermediate. Then water was involved to break the peptide bond and produced the tetrapeptide as product. Meanwhile, four amino acid residues (K80, Y171, N173 and D245) acted synergistically to stabilize the substrate and intermediate transition states. This study firstly revealed the enzymatic mechanism of MlrB for biodegrading linearized MCs with both computer simulation and experimental verification.

摘要

微囊藻毒素（MCs）是富营养化水体中分布最广泛的蓝藻毒素。作为高毒性中间代谢产物，线性化微囊藻毒素可被USTB - 05菌株的线性化微囊藻毒素酶（MlrB）进一步催化。在此，通过同源建模将MlrB结构与青霉素识别酶家族的模型代表进行比较研究。通过分子对接预测MlrB的关键活性位点，并通过定点诱变进一步验证。提出了MlrB对线性化微囊藻毒素进行生物降解的综合酶促机制：S77将一个质子转移至H307，以促进对线性化微囊藻毒素肽键（MC - LR中的Ala - Leu或MC - RR中的Ala - Arg）的亲核攻击，形成酰胺中间体。然后水参与断裂肽键并产生四肽作为产物。同时，四个氨基酸残基（K80、Y171、N173和D245）协同作用以稳定底物和中间过渡态。本研究首次通过计算机模拟和实验验证揭示了MlrB降解线性化微囊藻毒素的酶促机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4bf/11070527/e92f50c54298/fmicb-15-1389235-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4bf/11070527/b017fcfac6e5/fmicb-15-1389235-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4bf/11070527/0d57e0dbc21f/fmicb-15-1389235-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4bf/11070527/1c574182a7d0/fmicb-15-1389235-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4bf/11070527/91c1374c4678/fmicb-15-1389235-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4bf/11070527/80c49d1c44af/fmicb-15-1389235-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4bf/11070527/e92f50c54298/fmicb-15-1389235-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4bf/11070527/b017fcfac6e5/fmicb-15-1389235-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4bf/11070527/0d57e0dbc21f/fmicb-15-1389235-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4bf/11070527/1c574182a7d0/fmicb-15-1389235-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4bf/11070527/91c1374c4678/fmicb-15-1389235-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4bf/11070527/80c49d1c44af/fmicb-15-1389235-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4bf/11070527/e92f50c54298/fmicb-15-1389235-g006.jpg

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本文引用的文献

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Crystal structural analysis and characterization for MlrC enzyme of Sphingomonas sp. ACM-3962 involved in linearized microcystin degradation.
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Chemosphere. 2023 Mar;317:137866. doi: 10.1016/j.chemosphere.2023.137866. Epub 2023 Jan 12.
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In Silico Analyses and Cytotoxicity Study of Asiaticoside and Asiatic Acid from Malaysian Plant as Potential mTOR Inhibitors.马来植物中的积雪草苷和积雪草酸的计算机分析与细胞毒性研究作为潜在的 mTOR 抑制剂。
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