College of Bioengineering, Tianjin University of Science and Technology, Tianjin, 300457, China.
Guangdong Key Laboratory of Nanomedicine, CAS Key Laboratory of Health Informatics, Shenzhen Bioactive Materials Engineering Lab for Medicine, Institute of Biomedicine and Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.
Analyst. 2024 May 28;149(11):3064-3072. doi: 10.1039/d4an00189c.
Ratiometric near-infrared fluorescent pH probes with various p values were innovatively designed and synthesized based on cyanine with a diamine moiety. The photochemical properties of these probes were thoroughly evaluated. Among the series, IR-PHA exhibited an optimal p value of approximately 6.40, closely matching the pH of cancerous tissues. This feature is particularly valuable for real-time pH monitoring in both living cells and living mice. Moreover, when administered intravenously to tumor-bearing mice, IR-PHA demonstrated rapid and significant enhancement of near-infrared fluorescence and photoacoustic signals within the tumor region. This outcome underscores the probe's exceptional capability for dual-modal cancer imaging utilizing near-infrared fluorescence (NIRF) and photoacoustic (PA) modalities. Concurrently, the application of a continuous-wave near-infrared laser efficiently ablated cancer cells , attributed to the photothermal effect induced by IR-PHA. The results strongly indicate that IR-PHA is well-suited for NIRF/PA dual-modality imaging and photothermal therapy of tumors. This makes it a promising candidate for theranostic applications involving small molecules.
基于带有二胺部分的菁染料，创新性地设计和合成了具有各种 p 值的比率近红外荧光 pH 探针。这些探针的光化学性质得到了彻底的评估。在该系列中，IR-PHA 表现出约 6.40 的最佳 p 值，与癌组织的 pH 值非常匹配。这一特性对于实时监测活细胞和活体小鼠中的 pH 值特别有价值。此外，当静脉注射到荷瘤小鼠体内时，IR-PHA 在肿瘤区域内迅速显著增强近红外荧光和光声信号。这一结果突出了该探针利用近红外荧光（NIRF）和光声（PA）模式进行双模式癌症成像的卓越能力。同时，连续波近红外激光的应用有效地消融了癌细胞，这归因于 IR-PHA 诱导的光热效应。结果强烈表明，IR-PHA 非常适合用于 NIRF/PA 双模式成像和肿瘤光热治疗。这使其成为涉及小分子的治疗应用的有前途的候选者。
