Sepsis and Critical Illness Research Center, Department of Surgery, College of Medicine, Gainesville, Florida.
Department of Oral Biology, College of Dentistry, Gainesville, Florida.
Shock. 2024 Aug 1;62(2):208-216. doi: 10.1097/SHK.0000000000002379. Epub 2024 May 2.
Postsepsis early mortality is being replaced by survivors who experience either a rapid recovery and favorable hospital discharge or the development of chronic critical illness with suboptimal outcomes. The underlying immunological response that determines these clinical trajectories remains poorly defined at the transcriptomic level. As classical and nonclassical monocytes are key leukocytes in both the innate and adaptive immune systems, we sought to delineate the transcriptomic response of these cell types. Using single-cell RNA sequencing and pathway analyses, we identified gene expression patterns between these two groups that are consistent with differences in TNF-α production based on clinical outcome. This may provide therapeutic targets for those at risk for chronic critical illness in order to improve their phenotype/endotype, morbidity, and long-term mortality.
败血症后早期死亡率正被经历快速康复和有利出院的幸存者或发展为慢性重症疾病且预后不佳的幸存者所取代。决定这些临床轨迹的潜在免疫反应在转录组水平上仍未得到很好的定义。由于经典和非经典单核细胞是固有和适应性免疫系统中的关键白细胞，我们试图描绘这些细胞类型的转录组反应。使用单细胞 RNA 测序和途径分析，我们根据临床结果基于 TNF-α产生确定了这两组之间的基因表达模式。这可能为那些有发生慢性重症疾病风险的人提供治疗靶点，以改善他们的表型/亚型、发病率和长期死亡率。
