绵羊T细胞生长因子的初步特性及其对人、大鼠和小鼠细胞的种属限制性活性

Initial characterization of sheep T-cell growth factor and its species-restricted activity on human, rat, and mouse cells.

作者信息

English L S, Latta H, Whitehurst M

出版信息

Cell Immunol. 1985 Feb;90(2):314-21. doi: 10.1016/0008-8749(85)90196-0.

Abstract

Sheep T-cell growth factor (TCGF) was prepared from concanavalin A-activated sheep peripheral blood cells and subsequently characterized by ammonium sulfate precipitation, gel exclusion chromatography, and isoelectric focussing. The TCGF was found in the 60-80% ammonium sulfate fraction and was shown to have an apparent molecular weight of 32,500 and an isoelectric point in the range pI 5.2-5.5. The ability of the sheep TCGF to promote proliferation of activated human, sheep, mouse, and rat cells was compared with that of human TCGF prepared by phytohemagglutinin stimulation of lymphocytes from multiple donors and TCGF prepared from concanavalin A-stimulated rat and mouse spleen cells. Human TCGF was found to act across all species barriers, rat TCGF supported the growth of cells of all species except human, and mouse only promoted the growth of activated mouse and rat cells. Sheep TCGF was unique in being unable to support the growth of any cells except autologous cells.

摘要

绵羊T细胞生长因子（TCGF）由伴刀豆球蛋白A激活的绵羊外周血制备，随后通过硫酸铵沉淀、凝胶排阻色谱法和等电聚焦法进行表征。在硫酸铵含量为60%-80%的组分中发现了TCGF，其表观分子量为32,500，等电点在pI 5.2-5.5范围内。将绵羊TCGF促进活化的人、绵羊、小鼠和大鼠细胞增殖的能力，与通过植物血凝素刺激多个供体的淋巴细胞制备的人TCGF以及通过伴刀豆球蛋白A刺激大鼠和小鼠脾细胞制备的TCGF进行了比较。发现人TCGF可作用于所有物种屏障，大鼠TCGF支持除人以外所有物种细胞的生长，而小鼠TCGF仅促进活化的小鼠和大鼠细胞的生长。绵羊TCGF的独特之处在于，它只能支持自体细胞的生长，无法支持其他任何细胞的生长。