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通过宏转录组分析探索藏红花的病毒组。

Exploring the viral landscape of saffron through metatranscriptomic analysis.

机构信息

Instituto Botánico. Universidad de Castilla-La Mancha, Campus Universitario s/n, Albacete 02071, Spain.

Instituto Botánico. Universidad de Castilla-La Mancha, Campus Universitario s/n, Albacete 02071, Spain; Escuela Técnica Superior de Ingeniería Agronómica y de Montes y Biotecnología. Departamento de Ciencia y Tecnología Agroforestal y Genética. Universidad de Castilla-La Mancha, Albacete, Spain.

出版信息

Virus Res. 2024 Jul;345:199389. doi: 10.1016/j.virusres.2024.199389. Epub 2024 May 9.

DOI:10.1016/j.virusres.2024.199389
PMID:38714217
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11101869/
Abstract

Saffron (Crocus sativus L.), a historically significant crop valued for its nutraceutical properties, has been poorly explored from a phytosanitary perspective. This study conducted a thorough examination of viruses affecting saffron samples from Spanish cultivars, using high-throughput sequencing alongside a systematic survey of transcriptomic datasets from Crocus sativus at the Sequence Read Archive. Our analysis unveiled a broad diversity and abundance, identifying 17 viruses across the 52 analyzed libraries, some of which were highly prevalent. This includes known saffron-infecting viruses and previously unreported ones. In addition, we discovered 7 novel viruses from the Alphaflexiviridae, Betaflexiviridae, Potyviridae, Solemoviridae, and Geminiviridae families, with some present in libraries from various locations. These findings indicate that the saffron-associated virome is more complex than previously reported, emphasizing the potential of phytosanitary analysis to enhance saffron productivity.

摘要

藏红花(Crocus sativus L.)是一种具有重要历史意义的作物,因其具有营养特性而备受重视,但从植物检疫的角度来看,对其研究还很不完善。本研究采用高通量测序技术,同时对 Sequence Read Archive 中 Crocus sativus 的转录组数据集进行系统调查,对来自西班牙品种的藏红花样本中影响其的病毒进行了全面研究。我们的分析揭示了广泛的多样性和丰度,在分析的 52 个文库中鉴定出 17 种病毒,其中一些具有较高的流行率。其中包括已知感染藏红花的病毒和以前未报道过的病毒。此外,我们还从 Alphaflexiviridae、Betaflexiviridae、Potyviridae、Solemoviridae 和 Geminiviridae 家族发现了 7 种新型病毒,其中一些存在于来自不同地区的文库中。这些发现表明,与藏红花相关的病毒组比以前报道的更为复杂,强调了植物检疫分析提高藏红花产量的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64cd/11101869/a5c476e7f33e/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64cd/11101869/4592c0576168/gr1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64cd/11101869/40c8dba18262/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64cd/11101869/f271c6ca8162/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64cd/11101869/6859337b9140/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64cd/11101869/5f8f3a5359ee/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64cd/11101869/e3c66125ee87/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64cd/11101869/a5c476e7f33e/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64cd/11101869/4592c0576168/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64cd/11101869/1ed2aed4a960/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64cd/11101869/40c8dba18262/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64cd/11101869/f271c6ca8162/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64cd/11101869/6859337b9140/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64cd/11101869/5f8f3a5359ee/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64cd/11101869/e3c66125ee87/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64cd/11101869/a5c476e7f33e/gr8.jpg

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