用17β-羟基-17α-[3H]甲基-4,9,11-雌三烯-3-酮对雄激素受体进行光亲和标记。

Photoaffinity labelling of androgen receptors with 17 beta-hydroxy-17 alpha-[3H]methyl-4,9,11-estratrien-3-one.

作者信息

Brinkmann A O, Kuiper G G, de Boer W, Mulder E, van der Molen H J

出版信息

Biochem Biophys Res Commun. 1985 Jan 16;126(1):163-9. doi: 10.1016/0006-291x(85)90586-8.

Abstract

The synthetic androgen 17 beta-hydroxy-17 alpha-[3H]methyl-4,9,11-estratrien-3-one (R1881) has been used as photoaffinity label to characterize androgen receptors in calf uterus and rat prostate. Polyacrylamide gel electrophoresis under denaturing conditions showed that the DNA-binding form of the androgen receptor in calf uterus cytosol is a protein with a molecular mass of 98 kD. In rat prostate cytosol an androgen receptor with a molecular mass of 46 kD could be photoaffinity labelled with R1881. The photoaffinity labelling procedure described here provides a method for studying the hormone binding domain of androgen receptors in partial purified preparations.

摘要

合成雄激素17β-羟基-17α-[3H]甲基-4,9,11-雌甾三烯-3-酮（R1881）已被用作光亲和标记物，以鉴定小牛子宫和大鼠前列腺中的雄激素受体。变性条件下的聚丙烯酰胺凝胶电泳表明，小牛子宫胞质溶胶中雄激素受体的DNA结合形式是一种分子量为98 kD的蛋白质。在大鼠前列腺胞质溶胶中，分子量为46 kD的雄激素受体可用R1881进行光亲和标记。本文所述的光亲和标记程序提供了一种在部分纯化制剂中研究雄激素受体激素结合域的方法。