Goppelt M, Köhler L, Resch K
Biochim Biophys Acta. 1985 Mar 6;833(3):463-72. doi: 10.1016/0005-2760(85)90104-3.
Exogenous long-chain fatty acids are readily taken up by unstimulated lymphocytes derived from the thymus of calves or rabbits and esterified to complex lipids, primarily phospholipids and triacylglycerols. Compared to saturated fatty acids, unsaturated fatty acids are incorporated preferentially. Furthermore, unsaturated fatty acids are transferred from triacylglycerols to phospholipids. The transfer cannot be observed with palmitic acid. With regard to individual phospholipid species, oleic acid and linoleic acid are found primarily in phosphatidylcholine. Arachidonic acid, however, is transferred to phosphatidylethanolamine and phosphatidylinositol as well. This suggests an arachidonic-specific transfer between individual phospholipids. Stimulation of the cells with the mitogen concanavalin A results in an enhanced incorporation of the fatty acids and an enhanced transfer from triacylglycerols to phospholipids. Triacylglycerols may thus be regarded as a labile intracellular storage pool that may be activated upon mitogenic stimulation.
外源性长链脂肪酸很容易被源自小牛或兔子胸腺的未受刺激的淋巴细胞摄取，并酯化为复合脂质，主要是磷脂和三酰甘油。与饱和脂肪酸相比，不饱和脂肪酸优先被掺入。此外，不饱和脂肪酸从三酰甘油转移到磷脂。棕榈酸则未观察到这种转移。就个别磷脂种类而言，油酸和亚油酸主要存在于磷脂酰胆碱中。然而，花生四烯酸也会转移到磷脂酰乙醇胺和磷脂酰肌醇中。这表明在个别磷脂之间存在花生四烯酸特异性转移。用促有丝分裂原刀豆球蛋白A刺激细胞会导致脂肪酸掺入增加以及从三酰甘油到磷脂的转移增强。因此，三酰甘油可被视为一个不稳定的细胞内储存库，在有丝分裂刺激时可能被激活。
