Shandong Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou 253023, PR China; State Key Laboratory of Bioreactor Engineering, Department of Bioengineering, East China University of Science and Technology, 130 Meilong Rd, Shanghai 200237, PR China.
State Key Laboratory of Bioreactor Engineering, Department of Bioengineering, East China University of Science and Technology, 130 Meilong Rd, Shanghai 200237, PR China.
J Chromatogr A. 2024 Jun 21;1725:464876. doi: 10.1016/j.chroma.2024.464876. Epub 2024 Apr 12.
Herein, 2,4-dichlorophenoxyacetic acid (2,4-D) was used as a model template in a rational design strategy to produce water-compatible noncovalent imprinted microspheres. The proposed approach involved computational modelling for screening functional monomers and a simple method for preparing monodisperse and highly cross-linked microspheres. The fabricated non-imprinted polymer (NIP) and 2,4-d-imprinted polymer (2,4-d-MIP) were characterised, and their adsorption capabilities in an aqueous environment were evaluated. Results reveal that the pseudo-second-order kinetics model was appropriate for representing the adsorption of 2,4-D on NIP and 2,4-d-MIP, with R values of 0.97 and 0.99, respectively. The amount of 2,4-D adsorbed on 2,4-d-MIP (97.75 mg g) was considerably higher than those of phenoxyacetic acid (35.77 mg g), chlorogenic acid (9.72 mg g), spiramycin (1.56 mg g) and tylosin (1.67 mg g). Furthermore, it exhibited strong resistance to protein adsorption in an aqueous medium. These findings confirmed the feasibility of the proposed approach, providing a reference for the development of water-compatible noncovalent imprinted polymers.
在此，我们以 2,4-二氯苯氧乙酸（2,4-D）为模型模板，采用合理的设计策略制备了亲水性非共价印迹微球。该方法涉及功能单体的计算模型筛选以及制备单分散、高交联微球的简单方法。对制备的非印迹聚合物（NIP）和 2,4-二印迹聚合物（2,4-d-MIP）进行了表征，并评估了它们在水相环境中的吸附能力。结果表明，拟二级动力学模型适用于表示 2,4-D 在 NIP 和 2,4-d-MIP 上的吸附，其相关系数（R）分别为 0.97 和 0.99。2,4-d-MIP 上吸附的 2,4-D 量（97.75 mg g）明显高于苯氧乙酸（35.77 mg g）、绿原酸（9.72 mg g）、螺旋霉素（1.56 mg g）和泰乐菌素（1.67 mg g）。此外，它在水介质中表现出对蛋白质吸附的强抵抗力。这些发现证实了所提出方法的可行性，为开发亲水性非共价印迹聚合物提供了参考。
