School of Urban Construction, Changzhou University, Changzhou, 213164, China.
College of Resources and Environment, Chengdu University of Information Technology, Chengdu, 610225, China.
Chemosphere. 2024 Jul;359:142269. doi: 10.1016/j.chemosphere.2024.142269. Epub 2024 May 6.
Temperature is a significant operational parameter of denitrifying filter (DF), which affects the microbial activity and the pollutants removal efficiency. This study investigated the influence of temperature on performance of advanced synergistic nitrogen removal (ASNR) of partial-denitrification anammox (PDA) and denitrification, consuming the hydrolytic and oxidation products of refractory organics in the actual secondary effluent (SE) as carbon source. When the test water temperature (TWT) was around 25, 20, 15 and 10 °C, the filtered effluent total nitrogen (TN) was 1.47, 1.70, 2.79 and 5.52 mg/L with the removal rate of 93.38%, 92.25%, 87.33% and 74.87%, and the effluent COD was 8.12, 8.45, 10.86 and 12.29 mg/L with the removal rate of 72.41%, 66.17%, 57.35% and 51.87%, respectively. The contribution rate of PDA to TN removal was 60.44%∼66.48%, and 0.77-0.96 mg chemical oxygen demand (COD) was actually consumed to remove 1 mg TN. The identified functional bacteria, such as anammox bacteria, manganese oxidizing bacteria (MnOB), hydrolytic bacteria and denitrifying bacteria, demonstrated that TN was removed by the ASNR, and the variation of the functional bacteria along the DF layer revealed the mechanism of the TWT affecting the efficiency of the ASNR. This technique presented a strong adaptability to the variation of the TWT, therefore, it has broad application prospect and superlative application value in advanced nitrogen removal of municipal wastewater.
温度是反硝化滤池（DF）的重要运行参数，它会影响微生物活性和污染物去除效率。本研究考察了温度对部分反硝化氨氧化（PDA）和反硝化协同脱氮（ASNR）性能的影响，该工艺以实际二级出水（SE）中难生物降解有机物的水解和氧化产物为碳源。当试验水温（TWT）约为 25、20、15 和 10°C 时，过滤后的总氮（TN）分别为 1.47、1.70、2.79 和 5.52mg/L，去除率分别为 93.38%、92.25%、87.33%和 74.87%，出水 COD 分别为 8.12、8.45、10.86 和 12.29mg/L，去除率分别为 72.41%、66.17%、57.35%和 51.87%。PDA 对 TN 的去除贡献率为 60.44%∼66.48%，实际消耗 0.77-0.96mgCOD 去除 1mgTN。鉴定的功能细菌，如氨氧化菌、锰氧化菌（MnOB）、水解菌和反硝化菌，证明 TN 是通过 ASNR 去除的，DF 层中功能细菌的变化揭示了 TWT 影响 ASNR 效率的机制。该技术对 TWT 的变化具有很强的适应性，因此，它在城市污水的高级脱氮中具有广阔的应用前景和卓越的应用价值。
