Zhengzhou Key Laboratory of Criminal Science and Technology, Department of Criminal Science and Technology, Railway Police College, Zhengzhou, China.
College of Chemistry, Zhengzhou University, Zhengzhou, China.
Luminescence. 2024 May;39(5):e4769. doi: 10.1002/bio.4769.
Fluorene nucleus derivatives show great potential for building outstanding fluorescence probes. In this paper, a novel fluorescent probe was developed by reacting with fluorene core with azacyclobutane, which exhibits typical solvation chromogenic effect in solvent. The fluorescence of the probe quenched in highly polar solvent. Based on this phenomenon, a novel fluorescence system for trace water was constructed. The response of this probe was fast (30 s) and sensitive for the detection of trace water in organic solvents, and the detection limit of water content in DMSO reached 0.13%. In addition, the probe can also be made as a test strip combined with homemade portable device and a smartphone for rapid detection of trace water. The luminescence mechanism of the probe is theoretically calculated based on time-contained density functional theory (TDDFT). To showcase its practicality, it has been applied for the detection of trace water in honey and alcohol by dipstick. This method provides a new idea for designing efficient fluorescent probes based on dipstick and mobile phone rapid detection.
芴核衍生物在构建出色的荧光探针方面显示出巨大的潜力。本文通过将芴核与氮杂环丁烷反应，合成了一种新型荧光探针，该探针在溶剂中表现出典型的溶剂致变色效应。探针在高极性溶剂中的荧光被猝灭。基于这一现象，构建了一种用于痕量水的新型荧光体系。该探针的响应速度快（30s），对有机溶剂中痕量水的检测具有较高的灵敏度，在 DMSO 中的水含量检测限达到 0.13%。此外，该探针还可以与自制的便携式设备和智能手机结合制成试条，用于快速检测痕量水。基于含时密度泛函理论（TDDFT）对探针的发光机理进行了理论计算。为了展示其实际应用，该探针已用于检测蜂蜜和酒精中的痕量水。该方法为基于试条和手机快速检测设计高效荧光探针提供了新的思路。
