Wakasugi H, Dokhelar M C, Garson D, Harel-Bellan A, Fradelizi D, Tursz T
Eur J Immunol. 1985 Mar;15(3):256-61. doi: 10.1002/eji.1830150309.
The accessory function (AF) of various B cell lines has been investigated by studying their ability to replace monocytes inducing the proliferation of highly purified T lymphocytes in the presence of phytohemagglutinin. AF could be exerted by B cells from different origin, including Epstein-Barr virus (EBV) transformed lymphoblastoid cell lines (LCL), Burkitt's lymphoma (BL)-derived lines, either EBV-positive or not, pre-B and B leukemias and lymphomas. The EBV-converted BJAB-B95 BL cells could exert an AF as efficient as BJAB cells, their EBV-negative counterparts, which demonstrates that EBV itself does not play any role in this function. The HLA-DR antigen-negative BL-K562 hybrids PUTKO and DUTKO, T51.4.1.6 cells which derived from an HLA-DR-negative variant of the T51 LCL and 721.84.5 cells from a mutagenically dissected HLA-DR-negative clone of the 721 LCL, also exerted very efficient AF. 721 LCL, PUTKO and DUTKO hybrids could produce interleukin 1 activity when triggered with 12-O-tetradecanoyl-phorbol 13-acetate (TPA). Furthermore, addition of anti-DR monoclonal antibodies which blocked completely the AF of the B cell lines in mixed lymphocyte-tumor cell reaction did not affect the mitogen-triggered AF exerted by the same B cell lines. The induction of cellular differentiation with TPA markedly reduced the AF exerted by all B cell lines studied as well as by the hybrid cells DUTKO. By contrast, the PUTKO hybrid was unsensitive to sodium butyrate and TPA treatments, which were unable to abrogate its AF. Taken together, these data indicate that the AF in mitogen-induced T lymphocyte responses is dependent upon some precise maturational stages of accessory cells and HLA-DR antigen expression is not required for AF in mitogen-induced proliferation assays.
通过研究各种B细胞系在植物血凝素存在的情况下替代单核细胞诱导高度纯化的T淋巴细胞增殖的能力，对其辅助功能（AF）进行了研究。AF可由不同来源的B细胞发挥，包括爱泼斯坦-巴尔病毒（EBV）转化的淋巴母细胞系（LCL）、伯基特淋巴瘤（BL）衍生系（无论是否为EBV阳性）、前B细胞和B细胞白血病及淋巴瘤。EBV转化的BJAB-B95 BL细胞能够发挥与BJAB细胞（其EBV阴性对应物）一样高效的AF，这表明EBV本身在该功能中不起任何作用。HLA-DR抗原阴性的BL-K562杂交细胞PUTKO和DUTKO、源自T51 LCL的HLA-DR阴性变体的T51.4.1.6细胞以及源自721 LCL的诱变剖析的HLA-DR阴性克隆的721.84.5细胞，也发挥了非常高效的AF。721 LCL、PUTKO和DUTKO杂交细胞在用12-O-十四酰佛波醇13-乙酸酯（TPA）触发时可产生白细胞介素1活性。此外，在混合淋巴细胞-肿瘤细胞反应中，添加完全阻断B细胞系AF的抗DR单克隆抗体，并不影响同一B细胞系由丝裂原触发的AF。用TPA诱导细胞分化显著降低了所有研究的B细胞系以及杂交细胞DUTKO发挥的AF。相比之下，PUTKO杂交细胞对丁酸钠和TPA处理不敏感，这些处理无法消除其AF。综上所述，这些数据表明丝裂原诱导的T淋巴细胞反应中的AF取决于辅助细胞的某些精确成熟阶段，并且在丝裂原诱导的增殖试验中AF不需要HLA-DR抗原表达。
