Department and Graduate Institute of Microbiology and Immunology, National Defense Medical Center, Taipei, 11490, Taiwan; Institute of Preventive Medicine, National Defense Medical Center, New Taipei City, 23742, Taiwan; Department of Biology and Anatomy, National Defense Medical Center, Taipei, 11490, Taiwan.
Institute of Preventive Medicine, National Defense Medical Center, New Taipei City, 23742, Taiwan; Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, 11490, Taiwan.
Talanta. 2024 Aug 15;276:126215. doi: 10.1016/j.talanta.2024.126215. Epub 2024 May 6.
Antibody detection is the critical first step for tracking the spread of many diseases including COVID-19. Lateral flow immunoassay (LFIA) is the most commonly used method for rapid antibody detection because it is easy-to-use and inexpensive. However, LFIA has limited sensitivity when gold nanoparticles (AuNPs) are used as the signals. In this study, the endospores of Bacillus subtilis were used in combination with AuNP in a LFIA to detect antibodies. The endospores serve as a signal amplifier. The detection limit was about 10 M for anti-beta galactosidase antibody detection whereas the detection limit of conventional LFIA is about 10 M. Furthermore, the proposed methods have no additional user steps compared with the traditional LFIA. This method, therefore, improved the sensitivity 100-fold without compromising any advantages of LFIA. We believe that the proposed method will be useful for detection of antibodies against HIV, Zika virus, SARS-CoV-2, and so on.
抗体检测是追踪许多疾病（包括 COVID-19）传播的关键第一步。侧向流动免疫分析（LFIA）是用于快速抗体检测的最常用方法，因为它易于使用且价格低廉。然而，当金纳米粒子（AuNPs）用作信号时，LFIA 的灵敏度有限。在这项研究中，枯草芽孢杆菌的芽孢与 AuNP 一起用于 LFIA 中以检测抗体。芽孢作为信号放大器。检测限约为 10 M，用于检测抗β半乳糖苷酶抗体，而传统 LFIA 的检测限约为 10 M。此外，与传统 LFIA 相比，所提出的方法没有额外的用户步骤。因此，该方法在不影响 LFIA 任何优势的情况下将灵敏度提高了 100 倍。我们相信，该方法将有助于检测针对 HIV、寨卡病毒、SARS-CoV-2 等的抗体。
