Levinson A I, Hoxie J A, Matthews D M, Schreiber A D, Negendank W G
J Clin Lab Immunol. 1985 Jan;16(1):23-6.
To determine whether or not recently reported imbalances in putative immunoregulatory subsets of T-cells are related to impaired B-cell function in patients with multiple myeloma, we enumerated the level of T-lymphocyte subsets in and the pokeweed mitogen induced B-cell differentiation responses of blood mononuclear cells obtained from 13 patients. T-cell subsets were enumerated with the monoclonal antibodies OKT3 (peripheral T cells), OKT4 (helper/inducer cells) and OKT8 (suppressor/cytotoxic cells) using the Ortho Spectrum III fluorescence analyzer. B-cell differentiation was assessed with a reverse hemolytic plaque assay to enumerate immunoglobulin secreting cells in pokeweed mitogen stimulated cultures. Compared to controls, patients showed reduced percentages of OKT4 cells, increased percentages of OKT8 cells, and reduced OKT4/OKT8 ratios. Pokeweed mitogen induced responses were heterogeneous, but markedly depressed in 5/13 patients (hyporesponders). The percentages of OKT4 and OKT8 lymphocytes and OKT4/OKT8 ratios were similar in PWM responders and hyporesponders. Furthermore, there was no correlation between the ratio and the magnitude of the PWM response in individual patients. The data suggest that imbalances in putative immunoregulatory subsets of T cells, although common in multiple myeloma, are not likely a primary cause of impaired in vitro polyclonal B cell responses seen in this disease.
为了确定最近报道的T细胞假定免疫调节亚群失衡是否与多发性骨髓瘤患者B细胞功能受损有关，我们对13例患者血液单核细胞中的T淋巴细胞亚群水平以及商陆有丝分裂原诱导的B细胞分化反应进行了计数。使用Ortho Spectrum III荧光分析仪，用单克隆抗体OKT3（外周T细胞）、OKT4（辅助/诱导细胞）和OKT8（抑制/细胞毒性细胞）对T细胞亚群进行计数。通过反向溶血空斑试验评估B细胞分化，以计数商陆有丝分裂原刺激培养物中分泌免疫球蛋白的细胞。与对照组相比，患者OKT4细胞百分比降低，OKT8细胞百分比升高，OKT4/OKT8比值降低。商陆有丝分裂原诱导的反应存在异质性，但13例患者中有5例（低反应者）明显受到抑制。PWM反应者和低反应者中OKT4和OKT8淋巴细胞的百分比以及OKT4/OKT8比值相似。此外，个体患者中该比值与PWM反应的幅度之间没有相关性。数据表明，T细胞假定免疫调节亚群的失衡虽然在多发性骨髓瘤中很常见，但不太可能是该疾病中体外多克隆B细胞反应受损的主要原因。
