产 NDM-1 斯氏不动杆菌全质粒分析及其在几种不动杆菌属中的适应性。
Whole-plasmid analysis of NDM-1-producing Acinetobacter seifertii isolate and its fitness in several Acinetobacter species.
机构信息
Department of Microbiology and Samsung Medical Center, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea.
Department of Microbiology and Samsung Medical Center, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea.
出版信息
J Glob Antimicrob Resist. 2024 Sep;38:223-226. doi: 10.1016/j.jgar.2024.05.003. Epub 2024 May 7.
OBJECTIVES
The aim of this study was to characterize an NDM-1-producing Acinetobacter seifertii isolates from a patient in South Korea.
METHODS
Antibiotic susceptibility testing and genotyping using multigene sequencing were performed and whole plasmid sequences were determined.
RESULTS
The genotype of A. seifertii was ST1899 and was resistant to ceftazidime, trimethoprim-sulfamethoxazole, and piperacillin-tazobactam, in addition to carbapenem. bla was surrounded by the ISAba125 insertion sequence within the structure of Tn125 in the 47 kb-sized plasmid. The plasmid exhibited a structure similar to that of other plasmids of diverse Acinetobacter sp. found worldwide. Transconjugation and the growth curve indicated that the plasmid was adapted to A. seifertii rather than other closely related Acinetobacter sp.
CONCLUSIONS
Acquisition of carbapenem resistance by horizontal transfer of the bla-carrying plasmid from another Acinetobacter species was found with no growth defect.
目的
本研究旨在对来自韩国患者的产 NDM-1 型鲍曼不动杆菌进行特征分析。
方法
采用多位点基因序列分析进行药敏试验和基因分型，并确定全质粒序列。
结果
鲍曼不动杆菌的基因型为 ST1899，除碳青霉烯类外，还对头孢他啶、复方磺胺甲噁唑和哌拉西林他唑巴坦耐药。bla 被 ISAba125 插入序列包围在 Tn125 结构内，位于 47kb 大小的质粒中。该质粒表现出与来自世界各地的不同鲍曼不动杆菌属的其他质粒相似的结构。接合转移和生长曲线表明，该质粒适应于鲍曼不动杆菌，而不是其他密切相关的不动杆菌属。
结论
发现来自其他鲍曼不动杆菌属的 bla 携带质粒通过水平转移获得碳青霉烯类耐药性，且没有生长缺陷。
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