Department of Water and Wastewater Engineering, School of Urban Construction, Wuhan University of Science and Technology, Wuhan 430065, China.
Department of Water and Wastewater Engineering, School of Urban Construction, Wuhan University of Science and Technology, Wuhan 430065, China; Hubei Provincial Engineering Research Center of Urban Regeneration, Wuhan University of Science and Technology, Wuhan 430065, China.
Bioresour Technol. 2024 Jun;402:130816. doi: 10.1016/j.biortech.2024.130816. Epub 2024 May 7.
Current biological wastewater treatment processes usually have a drawback of insufficient nitrogen (N) removal, contributing to the ubiquitous eutrophication of aquatic ecosystems globally. To address such a challenging situation, this study explored an innovative microalgal-bacterial granular sludge-marimo (MBGS-MA) coupling process. The process removed 83.4 % of N with the effluent N concentration of 4.0 mg/L. With the growth of MBGS, there was a shift towards genes associated with nitrification and denitrification, and away from ammonia assimilation genes, revealing internal mechanism of the shift of N removal pathway. Contrarily, MA could use gaseous N with the N fixing genes in MA enriched, and the genes abundance related to assimilatory nitrate reduction were also raised under the mutualistic interactions between Proteobacteria and Cyanobacteria, which was beneficial to achieve efficient N removal. These findings may open a new horizon for developing innovative hybrid microalgal-bacterial processes aimed at high-efficiency N removal from wastewater.
当前的生物废水处理工艺通常存在氮（N）去除不足的缺点，导致全球水生生态系统普遍富营养化。为了解决这一具有挑战性的情况，本研究探索了一种创新的微藻-细菌颗粒污泥-藻球（MBGS-MA）耦合工艺。该工艺以 83.4%的去除率去除 N，出水 N 浓度为 4.0mg/L。随着 MBGS 的生长，与硝化和反硝化相关的基因发生转移，而与氨同化相关的基因则减少，揭示了 N 去除途径转变的内在机制。相反，藻球可以利用 MA 中富含固氮基因的气态 N，在 Proteobacteria 和 Cyanobacteria 之间的互利相互作用下，与同化硝酸盐还原相关的基因丰度也有所提高，这有利于实现高效的 N 去除。这些发现可能为开发旨在从废水中高效去除 N 的创新混合微藻-细菌工艺开辟新的前景。
