Berke G
Eur J Immunol. 1985 Apr;15(4):337-40. doi: 10.1002/eji.1830150406.
Using fluorescein-labeled effector lymphocytes and tetramethyl rhodamine-labeled target cells a cytofluorometric method is described for the detection and quantification of lymphocyte-target cell cluster (conjugate) formation. Conjugation levels measured by cytofluorography correspond well with those scored microscopically. The method has so far been used successfully to monitor conjugate formation by specifically sensitized cytotoxic T lymphocytes (CTL). In preliminary experiments it has been applied to nonspecific, lectin concanavalin A-dependent conjugate formation by CTL, as well as to conjugates formed by hybridoma CTL. The procedure has potential application in other cell-cell interactions, relevant to immunology as well as to cell biology.
利用荧光素标记的效应淋巴细胞和四甲基罗丹明标记的靶细胞，描述了一种细胞荧光测定法，用于检测和定量淋巴细胞-靶细胞簇（结合物）的形成。通过细胞荧光成像测量的结合水平与显微镜下评分的结果非常吻合。到目前为止，该方法已成功用于监测特异性致敏细胞毒性T淋巴细胞（CTL）的结合物形成。在初步实验中，它已应用于CTL的非特异性、凝集素伴刀豆球蛋白A依赖性结合物形成，以及杂交瘤CTL形成的结合物。该方法在与免疫学以及细胞生物学相关的其他细胞-细胞相互作用中具有潜在应用价值。
