Aldo-Benson M
Fed Proc. 1985 May;44(8):2493-6.
Studies of B cell tolerance at the single-cell level require a ready source of antigen-specific B cells that are uncontaminated by T cells or accessory cells. We have isolated normal dinitrophenyl (DNP)-specific B cells from spleens of unprimed mice and propagated these cells in vitro. These B cells are uncontaminated by T cells or macrophages. Long-term cultures of these cell lines contain pre-B cells that are surface (s) IgM-, B cells with sIgM alone, and more mature B cells with sIgM, sIgD, and Ia antigens. Using the cell line lymphocytes we have shown that the early binding of the tolerogenic form of hapten to B cell receptor on mature B cells induces the same activation signal as antigen, and the negative signal induced by tolerogen occurs after B cell activation. Exposure of maturing B cells to DNP bound to murine IgG2a (MGG) for 30 days does not inhibit growth or receptor expression, but does induce tolerance that is reversible when DNP-MGG is removed. A 45-day exposure to DNP-MGG also induces a reversible tolerance.
在单细胞水平上对B细胞耐受性的研究需要有未被T细胞或辅助细胞污染的抗原特异性B细胞的现成来源。我们从未免疫小鼠的脾脏中分离出正常的二硝基苯基（DNP）特异性B细胞，并在体外培养这些细胞。这些B细胞未被T细胞或巨噬细胞污染。这些细胞系的长期培养物中包含表面（s）IgM阴性的前B细胞、仅具有sIgM的B细胞以及具有sIgM、sIgD和Ia抗原的更成熟B细胞。利用这些细胞系淋巴细胞，我们已经表明，半抗原的致耐受性形式与成熟B细胞上的B细胞受体的早期结合诱导与抗原相同的激活信号，并且致耐受原诱导的负信号在B细胞激活后出现。将正在成熟的B细胞暴露于与鼠IgG2a（MGG）结合的DNP 30天不会抑制生长或受体表达，但会诱导耐受性，当去除DNP-MGG时这种耐受性是可逆的。暴露于DNP-MGG 45天也会诱导可逆的耐受性。
