School of Civil and Resource Engineering, University of Science and Technology Beijing, Beijing 100083, China; State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, China.
School of Civil and Resource Engineering, University of Science and Technology Beijing, Beijing 100083, China.
J Hazard Mater. 2024 Jul 5;472:134555. doi: 10.1016/j.jhazmat.2024.134555. Epub 2024 May 8.
This study aimed to isolate marine bacteria to investigate their stress response, inhibition mechanisms, and degradation processes under high-load conditions of salinity and enrofloxacin (ENR). The results demonstrated that marine bacteria exhibited efficient pollutant removal efficiency even under high ENR stress (up to 10 mg/L), with chemical oxygen demand (COD), total phosphorus (TP), total nitrogen (TN) and ENR removal efficiencies reaching approximately 88%, 83%, 61%, and 73%, respectively. The predominant families of marine bacteria were Bacillaceae (50.46%), Alcanivoracaceae (32.30%), and Rhodobacteraceae (13.36%). They responded to ENR removal by altering cell membrane properties, stimulating the activity of xenobiotic-metabolizing enzymes and antioxidant systems, and mitigating ENR stress through the secretion of extracellular polymeric substance (EPS). The marine bacteria exhibited robust adaptability to environmental factors and effective detoxification of ENR, simultaneously removing carbon, nitrogen, phosphorus, and antibiotics from the wastewater. The attapulgite carrier enhanced the bacteria's resistance to the environment. When treating actual mariculture wastewater, the removal efficiencies of COD and TN exceeded 80%, TP removal efficiency exceeded 90%, and ENR removal efficiency approached 100%, significantly higher than reported values in similar salinity reactors. Combining the constructed physical and mathematical models of tolerant bacterial, this study will promote the practical implementation of marine bacterial-based biotechnologies in high-loading saline wastewater treatment.
本研究旨在分离海洋细菌，以研究其在高盐度和恩诺沙星（ENR）负荷条件下的应激响应、抑制机制和降解过程。结果表明，海洋细菌即使在高 ENR 胁迫（高达 10 mg/L）下，也表现出高效的污染物去除效率，化学需氧量（COD）、总磷（TP）、总氮（TN）和 ENR 的去除效率分别达到约 88%、83%、61%和 73%。海洋细菌的主要科为芽孢杆菌科（50.46%）、噬烷菌科（32.30%）和红杆菌科（13.36%）。它们通过改变细胞膜特性、刺激外源性代谢酶和抗氧化系统的活性以及通过分泌胞外聚合物（EPS）来减轻 ENR 胁迫来应对 ENR 的去除。海洋细菌对环境因素表现出强大的适应性和对 ENR 的有效解毒能力，同时从废水中去除碳、氮、磷和抗生素。凹凸棒土载体增强了细菌对环境的抵抗力。在处理实际海水养殖废水时，COD 和 TN 的去除效率超过 80%，TP 的去除效率超过 90%，ENR 的去除效率接近 100%，明显高于类似盐度反应器中的报道值。结合耐受细菌的构建物理和数学模型，本研究将促进基于海洋细菌的生物技术在高负荷含盐废水处理中的实际应用。
