School of Food & Health, Jinzhou Medical University, Jinzhou, Liaoning 121001, China.
Liaoning Daoguang 25 Manchu Dynasty Wine Limited Liability Company, Jinzhou, Liaoning 121001, China.
Food Res Int. 2024 Jun;186:114319. doi: 10.1016/j.foodres.2024.114319. Epub 2024 Apr 18.
The "outstanding and unique aged aroma" of Chinese Chenxiang-type baijiu (CXB)-Daoguang 25 (DG25) mainly originates from a "extraordinary storage technology" of Mujiuhai (a wooden container), so it is mysterious and interesting. In this study, an untargeted GC/MS-based metabolomics was used to reveals the volatile differential metabolites for discriminating six different vintages of DG25 combing with chemometrics. A total of 100 volatile metabolites (including unknowns) were extracted and identified, including esters (41%), alcohols (10%) and acids (7%) so on. Finally, 33 differential metabolites were identified as aging-markers. Among them, 25 aging-markers showed a downtrend, including 17 esters such as ethyl acetate, ethyl hexanoate and ethyl palmitate so on. Moreover, it was interesting and to further study that furans showed a significant downtrend. Statistically speaking, ethyl benzoate played an important role in discriminating vintage of 1Y and 3Y, and the other 24 differential metabolites with downtrend discriminating the unstored (0Y-aged) DG25. Eight differential metabolites, such as ethyl octanoate, benzaldehyde, 3-methylbutanol and 1,1-diethoxyaccetal so on increased during aging of DG25, and they played a statistical role in discriminating the 5Y-, 10Y- and 20Y-aged DG25. This study provides a theoretical basis way for the formation mechanism of aging aroma for CXB.
中国陈香型白酒（CXB）-道光 25 年（DG25）的“卓越而独特的陈香”主要源自于木基容器“茅台酒”的“非凡储存技术”，因此它神秘而有趣。在本研究中，采用非靶向 GC/MS 代谢组学结合化学计量学方法，揭示了区分六个不同年份 DG25 的挥发性差异代谢物。共提取和鉴定了 100 种挥发性代谢物（包括未知物），包括酯类（41%）、醇类（10%）和酸类（7%）等。最后，确定了 33 种差异代谢物作为陈化标志物。其中，25 种陈化标志物呈下降趋势，包括乙酸乙酯、己酸乙酯和棕榈酸乙酯等 17 种酯类。此外，有趣的是，呋喃类物质呈显著下降趋势。从统计学上讲，苯甲酸乙酯在区分 1Y 和 3Y 年份方面起着重要作用，而其他 24 种呈下降趋势的差异代谢物则可区分未储存（0Y 陈化）的 DG25。8 种差异代谢物，如辛酸乙酯、苯甲醛、3-甲基-1-丁醇和 1,1-二乙氧基乙缩醛等，在 DG25 的陈化过程中增加，它们在区分 5Y、10Y 和 20Y 陈化的 DG25 方面具有统计学意义。本研究为 CXB 陈香形成机制提供了理论依据。
