Kottaridis S, Perez S, Kokkinopoulos D, Delinassios J G, Pangalis G A, Cosmidis H, Goula I, Papamichail M
Leuk Res. 1985;9(1):113-22. doi: 10.1016/0145-2126(85)90025-6.
A permanent lymphoblastoid cell line was established from the peripheral blood of a child with acute lymphoblastic leukemia. The cell line, designated SDK, grows in a stationary suspension culture, forming aggregates, in RPMI medium supplemented with 10% FCS, with a doubling time of 50-60 h. Immunologic markers and cytological features suggested that the SDK cells should be identified as being of B-cell origin. The cells failed to form rosettes with sheep erythrocytes, did not express T-cell antigens as defined by monoclonal antibodies, and exhibited surface and cytoplasmic immunoglobulin determinants. Chromosome analysis revealed the presence of three cell populations with (a) 46XY; (b) t(8q-; 14q+) or 2p-; 14q+) and (c) cells with unidentifiable markers. SDK demonstrated susceptibility to TPA-induced differentiation toward plasma cells.
从一名急性淋巴细胞白血病患儿的外周血中建立了一个永久性淋巴母细胞系。该细胞系命名为SDK，在添加10%胎牛血清的RPMI培养基中，以固定悬浮培养方式生长，形成聚集体，倍增时间为50 - 60小时。免疫标记和细胞学特征表明，SDK细胞应被鉴定为B细胞起源。这些细胞不能与绵羊红细胞形成玫瑰花结，不表达单克隆抗体所定义的T细胞抗原，并且表现出表面和细胞质免疫球蛋白决定簇。染色体分析显示存在三个细胞群体，(a) 46XY；(b) t(8q-; 14q+)或2p-; 14q+)以及(c)具有无法识别标记的细胞。SDK显示出对TPA诱导向浆细胞分化的敏感性。
