Jeromel Saša, Repše Fokter Alenka, Dovnik Andraž
Medical Faculty, University of Maribor, Taborska ulica 8, 2000 Maribor, Slovenia.
Department of Pathology and Cytology, General Hospital Celje, Oblakova 5, 3000 Celje, Slovenia.
Cancers (Basel). 2024 Apr 23;16(9):1621. doi: 10.3390/cancers16091621.
Very limited information exists about the role of p16/Ki67 dual staining on glandular cells in detecting glandular precancerous lesions and cervical adenocarcinoma. In this study, we investigated the diagnostic accuracy of p16/Ki67 dual staining for the detection of glandular and squamous lesions on the uterine cervix and for cancer of the upper reproductive tract. We performed a retrospective analysis of prospectively collected data on 96 patients with glandular cell abnormalities. We analyzed the diagnostic accuracy of p16/Ki67 dual staining for atypical glandular cells, not otherwise specified (AGC-NOS); atypical glandular cells, favor neoplastic (AGC-FN); adenocarcinoma in situ (AIS); and A-CA (cervical adenocarcinoma). A separate analysis for the detection of squamous precancerous lesions and squamous-cell carcinoma (CIN3+) and for cancer of the upper reproductive tract (EC/OC) was performed. Among patients who had normal histology or a low-grade lesion on final analysis, only 8.5% had positive dual staining. On the other hand, 85.7% of patients with AIS+ on final histology had positive dual staining. The respective specificities of p16/Ki67 dual staining on AGC-NOS for the detection of AIS+ (adenocarcinoma in situ or cervical adenocarcinoma), CIN3+ and EC/OC were 91.5%, 88.7% and 86.4%. High specificity values of p16/Ki67 dual staining on cervical smears labelled as AGC-NOS for the detection of CIN3+ and AIS+ suggest that this method might be a useful addition in cervical cancer screening.
关于p16/Ki67双重染色在检测腺上皮细胞癌前病变及宫颈腺癌中的作用，现有信息非常有限。在本研究中，我们调查了p16/Ki67双重染色对检测子宫颈腺性和鳞状病变以及上生殖道癌的诊断准确性。我们对前瞻性收集的96例腺上皮细胞异常患者的数据进行了回顾性分析。我们分析了p16/Ki67双重染色对未明确诊断的非典型腺上皮细胞（AGC-NOS）、倾向肿瘤性的非典型腺上皮细胞（AGC-FN）、原位腺癌（AIS）和宫颈腺癌（A-CA）的诊断准确性。对鳞状癌前病变和鳞状细胞癌（CIN3+）以及上生殖道癌（子宫内膜癌/卵巢癌）的检测进行了单独分析。在最终分析中组织学正常或为低级别病变的患者中，只有8.5%的患者双重染色呈阳性。另一方面，最终组织学诊断为AIS+的患者中，85.7%的患者双重染色呈阳性。p16/Ki67双重染色对AGC-NOS检测AIS+（原位腺癌或宫颈腺癌）、CIN3+和子宫内膜癌/卵巢癌的特异性分别为91.5%、88.7%和86.4%。p16/Ki67双重染色对标记为AGC-NOS的宫颈涂片检测CIN3+和AIS+具有较高的特异性值，表明该方法可能是宫颈癌筛查中一种有用的补充方法。
