Laforce Elien, Dejaeger Karlien, Vanoppen Marjolein, Cornelissen Emile, De Clercq Jeriffa, Vermeir Pieter
Industrial Catalysis and Adsorption Technology (INCAT), Department of Materials, Textiles and Chemical Engineering, Faculty of Engineering and Architecture, Ghent University, 9000 Ghent, Belgium.
PaInT, Particle and Interfacial Technology Group, Department of Green Chemistry and Technology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Ghent, Belgium.
Molecules. 2024 Apr 30;29(9):2075. doi: 10.3390/molecules29092075.
Size exclusion chromatography with total organic carbon detection (HPSEC-TOC) is a widely employed technique for characterizing aquatic natural organic matter (NOM) into high, medium, and low molecular weight fractions. This study validates the suitability of HPSEC-TOC for a simplified yet efficient routine analysis of freshwater and its application within drinking water treatment plants. The investigation highlights key procedural considerations for optimal results and shows the importance of sample preservation by refrigeration with a maximum storage duration of two weeks. Prior to analysis, the removal of inorganic carbon is essential, which is achieved without altering the NOM composition through sample acidification to pH 6 and subsequent N-purging. The chromatographic separation employs a preparative TSK HW-50S column to achieve a limit of detection of 19.0 µgC dm with an injection volume of 1350 mm. The method demonstrates linearity up to 10,000 µgC dm. Precision, trueness and recovery assessments are conducted using certified reference materials, model compounds, and real water samples. The relative measurement uncertainty in routine analysis ranges from 3.22% to 5.17%, while the measurement uncertainty on the bias is 8.73%. Overall, the HPSEC-TOC represents a reliable tool for NOM fractions analysis in both treated and untreated ground and surface water.
采用总有机碳检测的尺寸排阻色谱法（HPSEC-TOC）是一种广泛应用的技术，用于将水生天然有机物（NOM）表征为高、中、低分子量组分。本研究验证了HPSEC-TOC在淡水简化高效常规分析中的适用性及其在饮用水处理厂中的应用。该研究强调了获得最佳结果的关键程序注意事项，并表明了通过冷藏进行样品保存的重要性，最长保存期限为两周。在分析之前，去除无机碳至关重要，可通过将样品酸化至pH 6并随后进行氮气吹扫来实现，且不会改变NOM的组成。色谱分离采用制备型TSK HW-50S柱，进样体积为1350 mm时检测限为19.0 µgC dm。该方法在高达10,000 µgC dm的范围内具有线性。使用认证参考物质、模型化合物和实际水样进行精密度、准确性和回收率评估。常规分析中的相对测量不确定度范围为3.22%至5.17%，而偏差的测量不确定度为8.73%。总体而言，HPSEC-TOC是处理和未处理的地表水和地下水中NOM组分分析的可靠工具。
