Her Namguk, Amy Gary, Foss David, Cho Jaeweon, Yoon Yeomin, Kosenka Paul
Environmental Engineering, University of Colorado, Boulder 80309, USA.
Environ Sci Technol. 2002 Mar 1;36(5):1069-76. doi: 10.1021/es015505j.
High-performance liquid chromatography (HPLC)-size exclusion chromatography (SEC) with ultraviolet absorbance (UVA) and on-line dissolved organic carbon (DOC) detectors has been adapted and optimized under various conditions. An enhanced HPSEC-UVA system employing a modified commercially available DOC detector provides a better understanding of the qualitative and quantitative natural organic matter (NOM) properties in water samples by detecting aromatic and nonaromatic fractions of NOM as a function of molecular weight (MW). The most critical merit of this system is that the DOC detector is readily available and widely used. With only a few modifications, a commercially available TOC analyzer served as a DOC detector, integrated with the HPSEC to measure DOC along with UVA, and provided a specific UVA (SUVA) chromatograph that is useful information for drinking water plant design and operation. Without preconcentration, samples can be analyzed with a small amount of sample, with a DOC detection limit as low as 0.1 mg/L (as DOC).
配备紫外吸光度（UVA）和在线溶解有机碳（DOC）检测器的高效液相色谱（HPLC）-尺寸排阻色谱（SEC）已在各种条件下进行了调整和优化。一种采用改进的市售DOC检测器的增强型HPSEC-UVA系统，通过检测天然有机物（NOM）的芳香族和非芳香族组分随分子量（MW）的变化，能更好地了解水样中NOM的定性和定量特性。该系统最关键的优点是DOC检测器易于获得且广泛应用。只需进行一些修改，市售的总有机碳分析仪就可作为DOC检测器，与HPSEC集成以同时测量DOC和UVA，并提供特定紫外吸光度（SUVA）色谱图，这对饮用水厂的设计和运行是有用的信息。无需预浓缩，只需少量样品就能进行分析，DOC检测限低至0.1 mg/L（以DOC计）。
